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General Information
Symbol
Dmel\P{PTT-un}trolZCL1973
Species
D. melanogaster
Name
FlyBase ID
FBti0129821
Also Known As
trol-GFP
Description
Inserted Element
Uses
Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
LINE ID
Genomic Location
Chromosomal Location
X ( 3A3-3A4 )
Sequence Location
Target / Docking site
Member of Large Scale Dataset(s)
Description

Additional lines created; use of a high-throughput embryo sorter allowed screening of very large numbers of animals for GFP-expressing protein trap insertions.

A set of transgenic insertion stocks derived by TE mobilization using the protein-trap constructs P{PTT-GA}, P{PTT-GB}, and P{PTT-GC}. The constructs carry a w+mC mini-white visible marker and an Avic\GFP vital fluorescent protein-trap marker. In each of the three constructs, the splice acceptor and splice donor consensus sequences are in a different reading frame relative to the Avic\GFP sequence. For a successful GFP-positive insertion into an intron of a protein-coding gene, translation results in a fusion of the GFP to both the amino- and carboxyl-terminal parts of the trapped protein.

Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
3A3-3A4 (near gene of known cytology)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Location-dependent role
Size (Kb)
Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

trol reporter expression becomes visible around cardioblasts at embryonic stage 14. By stage 16, expression also surrounds pericardial nephrocytes and the surface of the lymph gland. Shortly after larval hatching, expression is observed covering each individual hematopoietic progenitor of the lymph gland. Expression is particularly strong around cells of the posterior signalling center. By the second instar stage, trol expression forms a solid layer around the surface of the primary and secondary lobes of the lymph gland. Within the lobes, expression is seen strongly around clusters of cells. The trol reporter pattern outlines a system of chambers that each contain small clusters of hematopoietic progenitors or differentiated hemocytes. Basement membrane components collagen and laminin are co-exexpressed with trol in the lymph gland. The structures containing trol reporter are revealed as basement membrane by electron microscopy.

GFP-tagged trol protein encoded by Avic\GFPtrol-ZCL1973 is first detected in the basement membrane surrounding cardioblasts at embryonic stage 14. By embryonic stage 16, tagged trol protein is associated with the basement membranes of pericardial nephrocytes, and at the surface of the embryonic/larval lymph gland. In newly hatched larvae, tagged trol protein surrounds hematopoietic progenitors of the lymph gland; expression is pronounced in cells of the posterior signalling center of the lymph gland primary lobe. In second and third instar larvae, tagged trol protein is concentrated in the medullary zone and expression is decreased in the cortical zone of lymph glands.

 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
P{PTT-un1}trolZCL1973
P{PTT-un}trolZCL1973
Name Synonyms
Secondary FlyBase IDs
    References (6)