FB2025_01 , released February 20, 2025
Insertion: Dmel\P{GawB}NP5272
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General Information
Symbol
Dmel\P{GawB}NP5272
Species
D. melanogaster
Name
FlyBase ID
FBti0130417
Feature type
transposable_element_insertion_site
Also Known As
NP5272-gal4, NP5272
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
Scer\GAL4
Viability / fertility
Associated allele(s)
Scer\GAL4NP5272
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
Sequence Location
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
P{GawB}
(Tanaka et al., 2008)
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene
Scer\GAL4
(Aso et al., 2012, Pauls et al., 2010, Tanaka et al., 2008)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
dopaminergic PAM neuron 3
(Flybrain Neuron Database et al., 2018.6.28, Shuai et al., 2015, Aso et al., 2012, Tanaka et al., 2008)
mushroom body beta lobe slice 2
(Shuai et al., 2015, Tanaka et al., 2008)
mushroom body beta lobe posterior layer
(Tanaka et al., 2008)
mushroom body beta lobe surface layer
(Tanaka et al., 2008)
adult dopaminergic PAM neuron | subset
(Aso et al., 2012)
adult brain | restricted
(Shuai et al., 2015)
mushroom body extrinsic neuron
(Shuai et al., 2015)
mushroom body beta' lobe slice 2
(Shuai et al., 2015)
Additional Information
Statement
Reference

ScerGAL4NP5272 drives expression in mushroom body extrinsic neurons, including mushroom body medial lobe arborizing neuron 3, that innervate mushroom body beta lobe slice 2 and mushroom body beta' lobe slice 2.

(Shuai et al., 2015)

ScerGAL4NP5272 drives expression in three dopaminergic PAM neurons (MB-M3 neurons).

(Aso et al., 2012)
 
Marker for
dopaminergic PAM neuron 3
(Tanaka et al., 2008)
Reflects expression of
Reporter construct used in assay
Mmus\Cd8aUAS.cLa.EGFP
(Aso et al., 2012, Tanaka et al., 2008)
Avic\GFPS65T.UAS
(Tanaka et al., 2008)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (15)