FlyBase Insertion Report: Dmel\P{GawB}NP0242

General Information

Symbol

Dmel\P{GawB}NP0242

Species

D. melanogaster

Name

FlyBase ID

FBti0130424

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^NP0242

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(Tanaka et al., 2008)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Pauls et al., 2010, Tanaka et al., 2008)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP0242

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

mushroom body output neuron 6

(Flybrain Neuron Database et al., 2018.6.28, Shuai et al., 2015, Tanaka et al., 2008)

mushroom body alpha' anterior-posterior layer

(Tanaka et al., 2008)

mushroom body alpha' lobe slice 1

(Tanaka et al., 2008)

mushroom body beta lobe posterior layer

(Tanaka et al., 2008)

mushroom body beta lobe surface layer

(Tanaka et al., 2008)

mushroom body beta lobe core layer

(Tanaka et al., 2008)

mushroom body beta lobe slice 1

(Shuai et al., 2015, Tanaka et al., 2008)

mushroom body beta lobe slice 2

(Shuai et al., 2015, Tanaka et al., 2008)

adult brain | restricted

(Shuai et al., 2015)

mushroom body extrinsic neuron

(Shuai et al., 2015)

mushroom body alpha lobe slice 1

(Shuai et al., 2015)

mushroom body alpha lobe slice 2

(Shuai et al., 2015)

mushroom body alpha lobe slice 3

(Shuai et al., 2015)

alpha/beta surface Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

alpha/beta posterior Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

alpha/beta core Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

Additional Information

Statement

Reference

ScerGAL4^NP0242 drives expression in mushroom body extrinsic neurons, including medial-vertical lobe arborizing neuron 2, that innervate mushroom body beta lobe slices 1 and 2, and mushroom body alpha lobe slices 1, 2, 3.

(Shuai et al., 2015)

Medial-vertical lobe arborizing neuron 2 arborisation within the beta lobe is within segment 1 and 2 of the posterior stratum, and within segment 1 of the middle and anterior strata.

(Tanaka et al., 2008)

Marker for

mushroom body output neuron 6

(Tanaka et al., 2008)

Reflects expression of

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Tanaka et al., 2008)

Avic\GFP^S65T.UAS

(Tanaka et al., 2008)

Data on Genetic Line

Line ID