A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\Mi{MIC}inscMI02180 Insertion

General Information
Symbol Dmel\Mi{MIC}inscMI02180 Species D. melanogaster
Name FlyBase ID FBti0141090
Feature type transposable_element_insertion_site
Description
Inserted element Mi{MIC} Expression data
Affected gene(s) insc, sktl Viability / fertility
Causes allele(s) inscMI02180, sktlMI02180 Stock availability 1 publicly available
LINE ID MI02180
Genomic Location
Chromosomal location 2R ( 57B3 ) Sequence location 2R:16,716,309..16,716,309 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0148058 FBti0141090 FBti0125331_1 FBti0125331_2 FBti0012555 FBti0026215 FBti0112383 FBti0105499 FBti0072112 FBti0005293 FBti0103927 FBti0049520 FBti0039972 FBti0066274 FBti0069556 FBti0026345 FBti0107498 FBti0054237 FBti0035892 FBti0035237 FBti0010767 FBti0054068 FBti0006238 FBti0113212 FBti0102522 FBti0072645 FBti0110065 FBti0069061 FBti0021886 FBti0034876 FBti0146211 FBti0102802 FBti0065445 FBti0065336 FBti0044344 FBti0065444 FBti0054314 FBti0036435 FBti0104612 FBti0103530 FBti0070061 FBti0102585 FBti0056006 FBti0035340
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Dhyd\Minos-based construct Mi{MIC}; created and vetted by the Gene Disruption Project (GDP). The Mi{MIC} construct carries the mini-yellow marker y[+mDint2] and Avic\GFP (a fluorescent marker) associated with a mutagenic gene-trap cassette. Two phiC31\attP sites are located immediately within the Dhyd\Minos termini, flanking the EGFP and yellow markers, allowing replacement with DNA of interest using Recombination Mediated Cassette Exchange (RMCE) methodology.
(Venken et al., 2011)
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Description
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FB2013_03
FB2013_02
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Chromosome (arm)
Sequence Location
2R:16,716,309..16,716,309 [-]
(Bellen, 2011.3.29)
Orientation
Cytological location
(computed by FlyBase)
57B3 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
57B3 (reported as inferred from sequence location)
(Bellen, 2011.3.29)
Comments concerning
location
hide Sequence Data
Flanking sequence
HR865248
(Bellen, 2011.3.29)
hide Inserted Element
Construct Mi{MIC}
Location-dependent
role
GFP gene trap
(Venken et al., 2009.12.19)
carries attP site(s)
(Venken et al., 2009.12.19)
Size 7.267, 7.267Kb
Associated alleles
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
insc
(Bellen, 2011.3.29)
sktl
(Bellen, 2011.3.29)
hide Alleles and Phenotypes
Causes alleles
inscMI02180
(Bellen, 2011.3.29)
sktlMI02180
(Bellen, 2011.3.29)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
MI02180
(Bellen, 2011.3.29)
Origin as a multiple insertion line
N
(Bellen, 2011.3.29)
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
35082
y1 w*; Mi{MIC}inscMI02180 sktlMI02180/SM6a
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
Mi{MIC}inscMI02180
(Bellen, 2011.3.29, )
Secondary FlyBase IDs
hide References ( 5 )
Research paper
Venken et al., 2011, Nat. Methods 8(9): 737--743
MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. [FBrf0216331]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Bellen, 2011.3.29, set930.xml
set930.xml [FBrf0213349]
Venken et al., 2009.12.19, MIMIC transposable element ("Minos Mediated Integration Casette")
MIMIC transposable element ("Minos Mediated Integration Casette") [FBrf0209655]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]