A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\Mi{MIC}CG42353MI02514 Insertion

General Information
Symbol Dmel\Mi{MIC}CG42353MI02514 Species D. melanogaster
Name FlyBase ID FBti0143063
Feature type transposable_element_insertion_site
Description
Inserted element Mi{MIC} Expression data
Affected gene(s) CG42353, CG42354 Viability / fertility
Causes allele(s) CG42353MI02514, CG42354MI02514 Stock availability 1 publicly available
LINE ID MI02514
Genomic Location
Chromosomal location X ( 14A9 ) Sequence location X:15,996,304..15,996,304 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0007545 FBti0039712 FBti0052972 FBti0036797 FBti0048793 FBti0143063 FBti0102456 FBti0046546 FBti0018796 FBti0071211 FBti0034600 FBti0102477 FBti0055210 FBti0035452 FBti0107702 FBti0056675 FBti0068555 FBti0065650 FBti0066924 FBti0047224 FBti0065188 FBti0043937
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Dhyd\Minos-based construct Mi{MIC}; created and vetted by the Gene Disruption Project (GDP). The Mi{MIC} construct carries the mini-yellow marker y[+mDint2] and Avic\GFP (a fluorescent marker) associated with a mutagenic gene-trap cassette. Two phiC31\attP sites are located immediately within the Dhyd\Minos termini, flanking the EGFP and yellow markers, allowing replacement with DNA of interest using Recombination Mediated Cassette Exchange (RMCE) methodology.
(Venken et al., 2011)
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Description
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FB2013_03
FB2013_02
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Chromosome (arm)
Sequence Location
X:15,996,304..15,996,304 [+]
(Bellen, 2011.5.24)
Orientation
Cytological location
(computed by FlyBase)
14A9 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
14A9 (reported as inferred from sequence location)
(Bellen, 2011.5.24)
Comments concerning
location
hide Sequence Data
Flanking sequence
JM029627
(Bellen, 2011.5.24)
hide Inserted Element
Construct Mi{MIC}
Location-dependent
role
GFP gene trap
(Venken et al., 2009.12.19)
carries attP site(s)
(Venken et al., 2009.12.19)
Size 7.267, 7.267Kb
Associated alleles
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG42353
(Bellen, 2011.5.24)
CG42354
(Bellen, 2011.5.24)
hide Alleles and Phenotypes
Causes alleles
CG42353MI02514
(Bellen, 2011.5.24)
CG42354MI02514
(Bellen, 2011.5.24)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
MI02514
(Bellen, 2011.5.24)
Origin as a multiple insertion line
N
(Bellen, 2011.5.24)
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
35994
y1 w* Mi{MIC}CG42353MI02514 CG42354MI02514
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hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
Mi{MIC}CG42353MI02514
(Bellen, 2011.5.24, )
Secondary FlyBase IDs
hide References ( 5 )
Research paper
Venken et al., 2011, Nat. Methods 8(9): 737--743
MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. [FBrf0216331]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Bellen, 2011.5.24, set932.xml
set932.xml [FBrf0213718]
Venken et al., 2009.12.19, MIMIC transposable element ("Minos Mediated Integration Casette")
MIMIC transposable element ("Minos Mediated Integration Casette") [FBrf0209655]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]