A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\Mi{MIC}Lk6MI02556 Insertion

General Information
Symbol Dmel\Mi{MIC}Lk6MI02556 Species D. melanogaster
Name FlyBase ID FBti0143071
Feature type transposable_element_insertion_site
Description
Inserted element Mi{MIC} Expression data
Affected gene(s) Lk6 Viability / fertility
Causes allele(s) Lk6MI02556 Stock availability 1 publicly available
LINE ID MI02556
Genomic Location
Chromosomal location 3R ( 86E18 ) Sequence location 3R:7,583,269..7,583,269 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0128326 FBti0066577 FBti0043158 FBti0143071 FBti0126040 FBti0103173 FBti0036610 FBti0105462 FBti0024863 FBti0105894 FBti0054798 FBti0034917 FBti0109144 FBti0103506 FBti0107742 FBti0144059 FBti0045474 FBti0106974 FBti0111262 FBti0110991 FBti0029245 FBti0112607 FBti0144077 FBti0047479 FBti0110178 FBti0028122 FBti0011488 FBti0111511 FBti0048313 FBti0029238 FBti0104335 FBti0105475 FBti0021481 FBti0104770 FBti0110514 FBti0103108 FBti0033463 FBti0058991 FBti0034616 FBti0114977 FBti0126041_2 FBti0126041_1 FBti0024389 FBti0103695 FBti0050079 FBti0045171 FBti0108710 FBti0024201 FBti0113143 FBti0103294 FBti0059192 FBti0070323 FBti0077083 FBti0029953 FBti0055030 FBti0011550 FBti0103753 FBti0057527 FBti0104315 FBti0111547 FBti0103691 FBti0059567 FBti0057882 FBti0058902 FBti0113329 FBti0108631 FBti0110350 FBti0112764 FBti0103403 FBti0034617 FBti0034705 FBti0030018 FBti0103139 FBti0111009 FBti0058881 FBti0054181 FBti0021139 FBti0029287 FBti0007862 FBti0008011 FBti0056154 FBti0011676 FBti0112490 FBti0100097 FBti0016513 FBti0011694 FBti0011390 FBti0016548 FBti0110947 FBti0011317 FBti0111318 FBti0033953 FBti0103164
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Dhyd\Minos-based construct Mi{MIC}; created and vetted by the Gene Disruption Project (GDP). The Mi{MIC} construct carries the mini-yellow marker y[+mDint2] and Avic\GFP (a fluorescent marker) associated with a mutagenic gene-trap cassette. Two phiC31\attP sites are located immediately within the Dhyd\Minos termini, flanking the EGFP and yellow markers, allowing replacement with DNA of interest using Recombination Mediated Cassette Exchange (RMCE) methodology.
(Venken et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:7,583,269..7,583,269 [+]
(Bellen, 2011.5.24)
Orientation
Cytological location
(computed by FlyBase)
86E18 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
86E18 (reported as inferred from sequence location)
(Bellen, 2011.5.24)
Comments concerning
location
hide Sequence Data
Flanking sequence
JM029635
(Bellen, 2011.5.24)
hide Inserted Element
Construct Mi{MIC}
Location-dependent
role
GFP gene trap
(Venken et al., 2009.12.19)
carries attP site(s)
(Venken et al., 2009.12.19)
Size 7.267, 7.267Kb
Associated alleles
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Lk6
(Bellen, 2011.5.24)
hide Alleles and Phenotypes
Causes alleles
Lk6MI02556
(Bellen, 2011.5.24)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
MI02556
(Bellen, 2011.5.24)
Origin as a multiple insertion line
N
(Bellen, 2011.5.24)
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
36001
y1 w*; Mi{MIC}Lk6MI02556
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hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
Mi{MIC}Lk6MI02556
(Bellen, 2011.5.24, )
Secondary FlyBase IDs
hide References ( 5 )
Research paper
Venken et al., 2011, Nat. Methods 8(9): 737--743
MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. [FBrf0216331]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Bellen, 2011.5.24, set932.xml
set932.xml [FBrf0213718]
Venken et al., 2009.12.19, MIMIC transposable element ("Minos Mediated Integration Casette")
MIMIC transposable element ("Minos Mediated Integration Casette") [FBrf0209655]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]