A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\Mi{MIC}AcslMI02966 Insertion

General Information
Symbol Dmel\Mi{MIC}AcslMI02966 Species D. melanogaster
Name FlyBase ID FBti0143223
Feature type transposable_element_insertion_site
Description
Inserted element Mi{MIC} Expression data
Affected gene(s) Acsl Viability / fertility
Causes allele(s) AcslMI02966 Stock availability 1 publicly available
LINE ID MI02966
Genomic Location
Chromosomal location 2R ( 44E1 ) Sequence location 2R:4,561,932..4,561,932 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0125036_2 FBti0125036_1 FBti0044551 FBti0103151 FBti0107722 FBti0109349 FBti0056156 FBti0112924 FBti0034872 FBti0028459 FBti0103452 FBti0039872 FBti0126403 FBti0036694 FBti0126497 FBti0066527 FBti0102710 FBti0103541 FBti0102214 FBti0033725 FBti0072171 FBti0026229 FBti0104237 FBti0108673 FBti0005299 FBti0107846 FBti0028917 FBti0112710 FBti0106796 FBti0105275 FBti0005991 FBti0106618 FBti0103625 FBti0104936 FBti0043909 FBti0102781 FBti0055536 FBti0016964 FBti0034269 FBti0103412 FBti0047829 FBti0143223 FBti0126439 FBti0050813
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Dhyd\Minos-based construct Mi{MIC}; created and vetted by the Gene Disruption Project (GDP). The Mi{MIC} construct carries the mini-yellow marker y[+mDint2] and Avic\GFP (a fluorescent marker) associated with a mutagenic gene-trap cassette. Two phiC31\attP sites are located immediately within the Dhyd\Minos termini, flanking the EGFP and yellow markers, allowing replacement with DNA of interest using Recombination Mediated Cassette Exchange (RMCE) methodology.
(Venken et al., 2011)
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Description
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FB2013_03
FB2013_02
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Chromosome (arm)
Sequence Location
2R:4,561,932..4,561,932 [-]
(Bellen, 2011.5.31)
Orientation
Cytological location
(computed by FlyBase)
44E1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
44E1 (reported as inferred from sequence location)
(Bellen, 2011.5.31)
Comments concerning
location
hide Sequence Data
Flanking sequence
JM113897
(Bellen, 2011.5.31)
hide Inserted Element
Construct Mi{MIC}
Location-dependent
role
GFP gene trap
(Venken et al., 2009.12.19)
carries attP site(s)
(Venken et al., 2009.12.19)
Size 7.267, 7.267Kb
Associated alleles
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Acsl
(Bellen, 2011.5.31)
hide Alleles and Phenotypes
Causes alleles
AcslMI02966
(Bellen, 2011.5.31)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
MI02966
(Bellen, 2011.5.31)
Origin as a multiple insertion line
N
(Bellen, 2011.5.31)
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
36173
y1 w*; Mi{MIC}AcslMI02966
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hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
Mi{MIC}AcslMI02966
(Bellen, 2011.5.31, )
Secondary FlyBase IDs
hide References ( 5 )
Research paper
Venken et al., 2011, Nat. Methods 8(9): 737--743
MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. [FBrf0216331]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Bellen, 2011.5.31, set938.xml
set938.xml [FBrf0213719]
Venken et al., 2009.12.19, MIMIC transposable element ("Minos Mediated Integration Casette")
MIMIC transposable element ("Minos Mediated Integration Casette") [FBrf0209655]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]