A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\Mi{MIC}Pde11MI02899 Insertion

General Information
Symbol Dmel\Mi{MIC}Pde11MI02899 Species D. melanogaster
Name FlyBase ID FBti0143477
Feature type transposable_element_insertion_site
Description
Inserted element Expression data
Affected gene(s) Pde11 Viability / fertility
Causes allele(s) Pde11MI02899 Stock availability 1 publicly available
LINE ID MI02899
Genomic Location
Chromosomal location 2L ( 36F6 ) Sequence location 2L:18,535,544..18,535,545
Map ( GBrowse ) GBrowse View Help detailed view FBti0152148 FBti0046595 FBti0143477 FBti0041575
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Dhyd\Minos-based construct Mi{MIC}; created and vetted by the Gene Disruption Project (GDP). The Mi{MIC} construct carries the mini-yellow marker y[+mDint2] and Avic\GFP (a fluorescent marker) associated with a mutagenic gene-trap cassette. Two phiC31\attP sites are located immediately within the Dhyd\Minos termini, flanking the EGFP and yellow markers, allowing replacement with DNA of interest using Recombination Mediated Cassette Exchange (RMCE) methodology.
(Venken et al., 2011)
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Description
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FB2013_03
FB2013_02
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Chromosome (arm)
Sequence Location
2L:18,535,544..18,535,545
(Levis, 2011.7.25)
Orientation
Cytological location
(computed by FlyBase)
36F6 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
36F6 (reported as inferred from sequence location)
(Bellen, 2011.5.31)
Comments concerning
location
The 5' and 3' flanking sequence maps to the same site but on opposite strands. The cause for this ambiguity is unknown, but possibilities include internal rearrangements within the transposon, or some kind of local inverted duplication of genomic sequence.
(Levis, 2011.7.25)
hide Sequence Data
Flanking sequence
JM113875
(Bellen, 2011.5.31)
JM113876
(Bellen, 2011.5.31)
hide Inserted Element
Construct
Location-dependent
role
Size
Associated alleles
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Pde11
(Bellen, 2011.5.31)
hide Alleles and Phenotypes
Causes alleles
Pde11MI02899
(Bellen, 2011.5.31)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
MI02899
(Bellen, 2011.5.31)
Origin as a multiple insertion line
P
(Bellen, 2011.5.31)
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
36256
y1 w*; Mi{MIC}Pde11MI02899
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
Mi{MIC}Pde11MI02899
 
Mi{MIC}Pde11MI02899a
(Bellen, 2011.5.31)
Mi{MIC}Pde11MI02899b
(Bellen, 2011.5.31)
Secondary FlyBase IDs
  • FBti0143201
  • FBti0143202
hide References ( 6 )
Research paper
Venken et al., 2011, Nat. Methods 8(9): 737--743
MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. [FBrf0216331]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Bellen, 2011.5.31, set938.xml
set938.xml [FBrf0213719]
Levis, 2011.7.25, Ambiguous sequence map data for four GDP lines.
Ambiguous sequence map data for four GDP lines. [FBrf0214586]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]