A set of transgenic insertion stocks derived by TE mobilization using one of the hybrid Tni\piggyBac-P-element protein-trap constructs PBac{566.P.SVS-1}, PBac{602.P.SVS-1}, PBac{681.P.FSVS-1}, PBac{754.P.FSVS-0}, PBac{802.P.SVS-2}, PBac{803.P.SVS-0}, or PBac{810.P.FSVS-2}. The constructs carry a w+mC mini-white visible marker and a Strep-tagged Avic\GFP vital fluorescent protein-trap marker (using the Venus YFP variant of GFP); P-element termini are nested inside PBac termini. The frame used for the splice acceptor and splice donor consensus sequences relative to the Avic\GFP sequence is indicated by 0, 1, or 2 in the construct symbol.
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AvicGFPRtnl1-CPTI001291 is expressed in larval epidermal cells overlapping with the ER retention marker KDEL. In the CNS, relatively low levels of AvicGFPRtnl1-CPTI001291 were found in cell bodies where most KDEL labelling is detected, much higher levels were found in the neuropil. In neuromuscular junctions AvicGFPRtnl1-CPTI001291 overlaps with the axonal microtubule marker futsch.
