A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{WH}Dskf02468b Insertion

General Information
Symbol Dmel\PBac{WH}Dskf02468b Species D. melanogaster
Name FlyBase ID FBti0147343
Feature type transposable_element_insertion_site
Description
Inserted element PBac{WH} Expression data
Affected gene(s) Dsk Viability / fertility
Causes allele(s) Dskf02468b Stock availability none publicly available
LINE ID
Genomic Location
Chromosomal location 3R ( 81F6 ) Sequence location
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
81F6 ( near gene of known cytology )
Cytological location
(reported)
Comments concerning
location
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct PBac{WH}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
mobile activating element
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.234Kb
Associated alleles
gypsy\su(Hw)BR348
Scer\UAS14x.hs.XP.Scer\FRT
Scer\UAS14x.hs.XP
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Dsk
(Chen and Ganetzky, 2012)
hide Alleles and Phenotypes
Causes alleles
Dskf02468b
(Chen and Ganetzky, 2012)
Lethality
References
Sterility
References
hide Phenotype Manifest In
embryonic/larval neuromuscular junction | third instar larval stage
(Chen and Ganetzky, 2012)
NMJ bouton | third instar larval stage
(Chen and Ganetzky, 2012)
hide Detailed Description
Statement
Reference
The neuromuscular junction (NMJ) undergrowth phenotype of homozygous Dsk[f02468b] larvae is rescued by Scer\GAL4[elav-C155]-driven expression of Dsk[Scer\UAS.cCa].
(Chen and Ganetzky, 2012)
Compared with wild-type, homozygous Dsk[GD6123] mutants exhibit mild neuromuscular junction (NMJ) undergrowth. Compared with wild-type, Dsk[GD6123]/Dsk[EP3632] mutants exhibit mild NMJ undergrowth. Compared with wild-type, hemizygous Dsk[f02468b]/Df(3R)2-2 larvae exhibit pronounced NMJ undergrowth. The NMJ undergrowth phenotypes of homozygous Dsk[f02468b] or hemizygous Dsk[f02468b]/Df(3R)2-2 larvae are rescued by Dp(3;1)2-2. Dsk[f02468b]/+ heterozygotes do not display NMJ) defects. The size and frequency of miniature excitatory junction potentials (mEJPs) from muscle 6 of Dsk[f02468b]/Df(3R)2-2 mutants does not differ from that of wild-type. However, the size of excitatory junction potentials (EJPs) is reduced in Dsk[f02468b]/Df(3R)2-2 mutants compared with wild-type.
(Chen and Ganetzky, 2012)
Whereas Df(1)Exel9051/+ alone or Dsk[f02468b]/+ heterozygotes alone do not display neuromuscular junction (NMJ) defects, Df(1)Exel9051/+; Dsk[f02468b]/+ double heterozygotes exhibit a strong reduction in synaptic bouton number. In contrast, heterozygosity for Dsk[f02468b] does not further enhance the NMJ undergrowth phenotype of Df(1)Exel9051/Y mutants. The NMJ undergrowth phenotype of homozygous Dsk[f02468b] is suppressed by Scer\GAL4[elav-C155]-driven overexpression of CCKLR-17D1[Scer\UAS.cCa].
(Chen and Ganetzky, 2012)
Under dim light conditions, the distance travelled by Dsk[f02468b]/Df(3R)2-2 third instar larvae in one minute at 20-22[o]C is slightly reduced compared to wild type. The frequency of peristaltic contractions under these conditions is also slightly reduced. Under bright light at 27-30[o]C (stress conditions) the mutant larvae travel significantly less far than wild type in one minute. Under these conditions, the frequency of peristaltic contractions is reduced in the mutant larvae compared to wild type.
(Chen et al., 2012)
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Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
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Aberration
Balancer
hide Stocks ( 0 )
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hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
dskf02468
(Chen and Ganetzky, 2012)
dskPB
(Chen and Ganetzky, 2012)
PBac{WH}Dskf02468b
(FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 6 )
Research paper
Chen and Ganetzky, 2012, J. Cell Biol. 196(4): 529--543
A neuropeptide signaling pathway regulates synaptic growth in Drosophila. [FBrf0217500]
Chen et al., 2012, Fly 6(4): 290--297
Drosulfakinin activates CCKLR-17D1 and promotes larval locomotion and escape response in Drosophila. [FBrf0220273]
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]