Dmel\P{Switch2}GSG8308 Insertion

General Information
Symbol	Dmel\P{Switch2}GSG8308	Species	D. melanogaster
Name		FlyBase ID	FBti0147958
Feature type	transposable_element_insertion_site
Description
Inserted element	P{Switch2}	Expression data	GAL4 reporter/driver
Affected gene(s)	mir-282, Scer\GAL4	Viability / fertility
Causes allele(s)	Scer\GAL4^GSG8308	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	3L ( 63C1 )	Sequence location	3L:3,250,565..3,250,700
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:3,250,565..3,250,700 (Cole et al., 2012.7.6)
Orientation
Cytological location (computed by FlyBase)	63C1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	63C1 (reported as inferred from sequence location) (Nicholson et al., 2008) 63C1_r4 (reported as inferred from sequence location) (Cole et al., 2012.7.6)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{Switch2}
Location-dependent role
Size	7.931, 7.931Kb
Associated alleles	Scer\GAL4^P\T.Switch2 w^+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene	mir-282 (Nicholson et al., 2008, Cole et al., 2012.7.6) Scer\GAL4 (Nicholson et al., 2008)
Alleles and Phenotypes
Causes alleles	Scer\GAL4^GSG8308 (Nicholson et al., 2008)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference third instar larval stage embryonic/larval neuron \| subset (Nicholson et al., 2008) ventral midline neuron \| subset of ventral nerve cord (Nicholson et al., 2008) midline neuron \| subset of ventral nerve cord \| dorsal (Nicholson et al., 2008) neuron \| subset of ventral nerve cord \| lateral (Nicholson et al., 2008) ipsilateral tract (Nicholson et al., 2008) embryonic/larval trachea (Nicholson et al., 2008) embryonic/larval oenocyte (Nicholson et al., 2008)
Additional Information
Statement Reference Scer\GAL4[GSG8308] drives primarily neuronal expression. Weak expression is observed in the ventral nerve cord at in dorsal midline, ventral midline, and a few lateral cells, and in lateral intersegmental processes. Strong expression is observed in a few cells in the brain. Strong expression is observed in the trachea and oenocytes. (Nicholson et al., 2008)

Marker for
Reflects expression of
Reporter construct used in assay	Avic\GFP^{2x.EGFP.Scer\UAS} (Nicholson et al., 2008)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	40329 y¹ w^*; P{Switch2}GSG8308
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	8308 (Nicholson et al., 2008, Cole et al., 2012.7.6) P{Switch2}GSG8308
Secondary FlyBase IDs

References ( 4 )
Supplementary material	Nicholson et al., 2008, Genetics 178(1): Supplemental Table 3. GeneSwitch GAL4 line expression patterns. [FBrf0218769]
Personal communication to FlyBase	Cole et al., 2012.7.6, Molecular mapping information for GeneSwitch lines from FBrf0202785. Molecular mapping information for GeneSwitch lines from FBrf0202785. [FBrf0219065]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]
DNA/RNA sequence record	Roman, 2002.6.25, GenBank/EMBL/DDBJ: AF524839 P-element cloning system vector pP{Switch2}, complete sequence. [FBrf0172537]