A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\Mi{MIC}HDAC4MI05513 Insertion

General Information
Symbol Dmel\Mi{MIC}HDAC4MI05513 Species D. melanogaster
Name FlyBase ID FBti0148536
Feature type transposable_element_insertion_site
Description
Inserted element Mi{MIC} Expression data
Affected gene(s) HDAC4 Viability / fertility
Causes allele(s) HDAC4MI05513 Stock availability 1 publicly available
LINE ID MI05513
Genomic Location
Chromosomal location X ( 11E8 ) Sequence location X:13,174,009..13,174,009 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0048396 FBti0048829 FBti0024998 FBti0054265 FBti0029694 FBti0034534 FBti0115016 FBti0039954 FBti0057002 FBti0099805 FBti0035513 FBti0029074 FBti0071366 FBti0049173 FBti0148536 FBti0071901 FBti0144061 FBti0128099 FBti0059038 FBti0108516 FBti0102492 FBti0052780 FBti0102474 FBti0056355 FBti0041610 FBti0075148
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Dhyd\Minos-based construct Mi{MIC}; created and vetted by the Gene Disruption Project (GDP). The Mi{MIC} construct carries the mini-yellow marker y[+mDint2] and Avic\GFP (a fluorescent marker) associated with a mutagenic gene-trap cassette. Two phiC31\attP sites are located immediately within the Dhyd\Minos termini, flanking the EGFP and yellow markers, allowing replacement with DNA of interest using Recombination Mediated Cassette Exchange (RMCE) methodology.
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Description
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FB2013_03
FB2013_02
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Chromosome (arm)
Sequence Location
X:13,174,009..13,174,009 [+]
Orientation
Cytological location
(computed by FlyBase)
11E8 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
11E8 (reported as inferred from sequence location)
Comments concerning
location
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Flanking sequence
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Construct Mi{MIC}
Location-dependent
role
GFP gene trap
carries attP site(s)
Size 7.267, 7.267Kb
Associated alleles
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
hide Alleles and Phenotypes
Causes alleles
Lethality
References
Sterility
References
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Statement
Reference
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Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
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Line ID
MI05513
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
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hide Comments
INSERTDATA:Mapped to HDAC4 intron by 5prime flank segment 100 bp away from insertion site. The ~100 bp segment of the 5prime flank adjacent to the insertion site does not align with D. melanogaster reference genomic sequence, but aligns with HDAC4 homolog in D. sechellia reference sequence
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Reported As
Symbol Synonym
Mi{MIC}HDAC4MI05513
Secondary FlyBase IDs
hide References ( 4 )
Research paper
Venken et al., 2011, Nat. Methods 8(9): 737--743
MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. [FBrf0216331]
Personal communication to FlyBase
Bellen, 2012.8.21, set1002.xml
set1002.xml [FBrf0219255]
Venken et al., 2009.12.19, MIMIC transposable element ("Minos Mediated Integration Casette")
MIMIC transposable element ("Minos Mediated Integration Casette") [FBrf0209655]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]