Dmel\P{UAS-CAG.99}cher^C Insertion

General Information
Symbol	Dmel\P{UAS-CAG.99}cher^C	Species	D. melanogaster
Name		FlyBase ID	FBti0148581
Feature type	transposable_element_insertion_site
Description
Inserted element	P{UAS-CAG.99}	Expression data
Affected gene(s)	cher	Viability / fertility
Causes allele(s)	cher^C	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 89E12-89F1 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	89E12-89F1 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{UAS-CAG.99}
Location-dependent role
Size
Associated alleles	Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}
Molecular map
Affected Gene(s)
Insertion may affect gene	cher (Lawlor et al., 2011)
Alleles and Phenotypes
Causes alleles	cher^C (Lawlor et al., 2011)

Lethality References
Sterility References
Phenotype Manifest In
	ommatidium (Lawlor et al., 2011)
Detailed Description
Statement Reference Expression of cher[C] in all cells of the eye under the control of Scer\GAL4[GMR.PF] results in a consistent mild disruption to the precise patterning of ommatidia on the exterior surface of the eye when compared to control lines. Neurodegeneration associated with this phenotype is confirmed in sections of aged adult eyes, where the number of rhabdomeres in each ommatidial unit decreased significantly from the normal number of seven observed in controls. A decrease in life span is observed in flies expressing cher[C] ubiquitously under the control of Scer\GAL4[da.G32]. An increase in the proportion of flies that fail to climb is observed when cher[C] is expressed throughout the nervous system using Scer\GAL4[elav-C155]. Flies expressing cher[C] throughout the nervous system also show a movement disorder involving a sudden loss of coordination in which flies will fall on their backs, followed by a brief period of uncoordinated movement in which they are not able to right themselves. This behaviour can also be induced by knocking flies to the bottom of the vial, and therefore may contribute to the observed failure to climb phenotype. The incidence of this phenotype is higher at 37[o]C compared with controls. (Lawlor et al., 2011)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-CAG.99}cher^C rCAG_~100<up>line C</up> (Lawlor et al., 2011, Lawlor et al., 2011)
Secondary FlyBase IDs

References ( 2 )
Research paper	Lawlor et al., 2011, Hum. Mol. Genet. 20(19): 3757--3768 Double-stranded RNA is pathogenic in Drosophila models of expanded repeat neurodegenerative diseases. [FBrf0215240]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{UAS-CAG.99}cherC Insertion

Dmel\P{UAS-CAG.99}cher^C Insertion