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General Information
Symbol
Dmel\P{PTT-un}vkgG00454
Species
D. melanogaster
Name
FlyBase ID
FBti0153267
Also Known As
Vkg-GFP, Viking-GFP, G454, Vkg::GFP, vkgG454.GFP
Description
Inserted Element
Uses
Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
LINE ID
Genomic Location
Chromosomal Location
2L ( 25C1 )
Sequence Location
2L:5,024,627..5,024,627 [-]
Target / Docking site
Member of Large Scale Dataset(s)
Description

Additional lines created; use of a high-throughput embryo sorter allowed screening of very large numbers of animals for GFP-expressing protein trap insertions.

A set of transgenic insertion stocks derived by TE mobilization using the protein-trap constructs P{PTT-GA}, P{PTT-GB}, and P{PTT-GC}. The constructs carry a w+mC mini-white visible marker and an Avic\GFP vital fluorescent protein-trap marker. In each of the three constructs, the splice acceptor and splice donor consensus sequences are in a different reading frame relative to the Avic\GFP sequence. For a successful GFP-positive insertion into an intron of a protein-coding gene, translation results in a fusion of the GFP to both the amino- and carboxyl-terminal parts of the trapped protein.

Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:5,024,627..5,024,627 [-]
Orientation
Cytological location (computed by FlyBase)
25C1 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location

Insertion reported at coordinate 84156 on AE003608.1.

Sequence Data
Flanking sequence
Inserted Element
Location-dependent role
Size (Kb)
Associated alleles
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Vkg::GFP protein localizes to the basal side of cardial cells.

Avic\GFPvkg-G00454 is localized to an intricate extracellular matrix network forming chamber-like structures within the cortical zone of the lymph gland.

 
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (25)
References (29)