A set of transgenic insertion stocks containing a protein trap cassette, derived from Mi{MIC} insertions in coding introns using Recombinase-Mediated Cassette Exchange (RMCE); see http://flypush.imgen.bcm.tmc.edu/pscreen/technique.html. The protein trap cassette contains a EGFP-FlAsH-StrepII-TEV-3xFlag ("GFSTF") tag that is flanked on either side with a 4X(GlyGlySer) flexible linker; the mini-yellow marker is no longer present. Each insertion line contains one of three versions of the cassette (Mi{PT-GFSTF.0}, Mi{PT-GFSTF.1} or Mi{PT-GFSTF.2}), depending on the reading frame required to generate an in-frame fusion protein with the gene in which the insertion is located. This system also allows UAS/GAL4-based knockdown strategies targeting GFP-sequence containing mRNA (RNAi against GFP) or GFP fusion protein (deGradFP).
MI06307-GFSTF.2
The parent MI06307 MiMIC line contains a single insertion, but the flanking sequence matches a duplicated region of the reference genome. Both flanks align equally well at two sites: 3L:19716031 (-) (release 6) in a coding intron of the Trpml gene and at 3L:19747447 (-) in a coding intron of the CG42638 gene and an intron in the 5' UTR of the CG42637 gene. Thus there are two possible locations for the parent MI06307 MiMIC line, 31kb apart and it is not known to which location the RMCE-derived Mi{PT-GFSTF.2}MI06307 line maps, or which of Trpml or CG42638 the Mi{PT-GFSTF.2}MI06307 line is predicted to protein trap.
Chromosome is homozygous lethal.