General Information
Symbol
Dmel\Mi{PT-GFSTF.0}taccMI03410-GFSTF.0
Species
D. melanogaster
Name
FlyBase ID
FBti0178600
Description
Inserted Element
Uses
Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
LINE ID
MI03410-GFSTF.0
Genomic Location
Chromosomal Location
3R ( 82D2 )
Sequence Location
3R:4,748,673..4,748,673 [+]
Target / Docking site
Member of Large Scale Dataset(s)
Description
A set of transgenic insertion stocks containing a protein trap cassette, derived from Mi{MIC} insertions in coding introns using Recombinase-Mediated Cassette Exchange (RMCE); see http://flypush.imgen.bcm.tmc.edu/pscreen/technique.html. The protein trap cassette contains a EGFP-FlAsH-StrepII-TEV-3xFlag ("GFSTF") tag that is flanked on either side with a 4X(GlyGlySer) flexible linker; the mini-yellow marker is no longer present. Each insertion line contains one of three versions of the cassette (Mi{PT-GFSTF.0}, Mi{PT-GFSTF.1} or Mi{PT-GFSTF.2}), depending on the reading frame required to generate an in-frame fusion protein with the gene in which the insertion is located. This system also allows UAS/GAL4-based knockdown strategies targeting GFP-sequence containing mRNA (RNAi against GFP) or GFP fusion protein (deGradFP).
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:4,748,673..4,748,673 [+]
Cytological location (computed by FlyBase)
82D2 (inferred by FlyBase from sequence location)
Cytological location (reported)
82D2_r6 (reported as inferred from sequence location)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Location-dependent role
Size (Kb)
Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene
Alleles and Phenotypes
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Linkouts
Gene Disruption Project - MiMIC RMCE Lines - A collection of protein trap lines made by converting MiMIC insertions by RMCE
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Mi{PT-GFSTF.0}taccMI03410-GFSTF.0
Name Synonyms
Secondary FlyBase IDs
    References (1)