FlyBase Insertion Report: Dmel\TI{GAL4}TrpA1-CD

General Information

Symbol

Dmel\TI{GAL4}TrpA1-CD

Species

D. melanogaster

Name

FlyBase ID

FBti0185284

Feature type

insertion_site

Also Known As

trpA1-CD^GAL4

Description

Inserted Element

TI{GAL4}

LINE ID

Affected gene(s)

TrpA1

Viability / fertility

Associated allele(s)

Scer\GAL4^TrpA1-CD-G4, TrpA1^-ACD-G4, TrpA1^-CD-G4

Stock availability

3 publicly available

Genomic Location

Chromosomal Location

3L ( 66E3 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

66E3 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

TI{GAL4}

(Sokabe et al., 2016)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

TrpA1

(Leung et al., 2020, Luo et al., 2017, Sokabe et al., 2016)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^TrpA1-CD-G4

Phenotypic Class Phenotype Manifest in Detailed Description

TrpA1^-ACD-G4

Phenotypic Class Phenotype Manifest in Detailed Description

TrpA1^-CD-G4

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval multidendritic class IV neuron

(Luo et al., 2017, Sokabe et al., 2016)

larval neuron of larval abdominal sensillum

(Sokabe et al., 2016)

Additional Information

Statement

Reference

ScerGAL4^TrpA1-CD-G4 drives expression under control of the promoter for the TrpA1-RG, TrpA1-RHand TrpA1-RK (TrpA1-C and TrpA1-D; upstream promoter) isoforms.

(Bloomington Drosophila Stock Center, 2017)

Positive GRASP (GFP reconstitution across synaptic partners) signals between class IV dendritic arborizing neurons labelled with ScerGAL4^TrpA1-CD-G4 and larval TrpA1 neurons labelled with EcollexA^{TrpA1-AB-lexA} were observed.

(Luo et al., 2017)

ScerGAL4^TrpA1-CD-G4 drives expression in class IV dendritic arborizing neurons and larval abdominal sensillum neurons in the body wall that extend axons to the ventral nerve cord.

(Sokabe et al., 2016)

Marker for

Reflects expression of

TrpA1

(Bloomington Drosophila Stock Center, 2017)

Reporter construct used in assay

Ecol\lexA^{TrpA1-AB-lexA}

(Luo et al., 2017)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome