The homology arms of the donor plasmid used in generating the TI{CRIMIC.GT14}EphCR00011 insertion were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion is predicted to be accompanied by a deletion of 13bp of genomic sequence flanking the insertion site.
The PCR check of the insertion gave the expected sized band for the left flank and a shorter than expected sized product for the right flank.
CR00011