FB2025_01 , released February 20, 2025
Insertion: Dmel\TI{CRIMIC.GT14}Idh3aCR00027
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General Information
Symbol
Dmel\TI{CRIMIC.GT14}Idh3aCR00027
Species
D. melanogaster
Name
FlyBase ID
FBti0200718
Feature type
insertion_site
Description
Inserted Element
TI{CRIMIC.GT14}
LINE ID

CR00027

Affected gene(s)
Idh3a
Viability / fertility
Associated allele(s)
Idh3aCR00027
Stock availability
None publicly available
Genomic Location
Chromosomal Location
X ( 18C8 )
Sequence Location
X:19,521,465..19,521,505 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
Description
Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:19,521,465..19,521,505 [-]
(Levis, 2018.8.30)
Orientation

-

(Levis, 2018.8.30)
Cytological location (computed by FlyBase)
18C8 (inferred by FlyBase from sequence location)
Cytological location (reported)
18C8_r6 (reported as inferred from sequence location)
(Levis, 2018.8.30)
Insertion into Natural transposon
Comments concerning location

The homology arms of the donor plasmid used in generating the TI{CRIMIC.GT14}Idh3aCR00027 insertion were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion is predicted to be accompanied by a deletion of 40bp of genomic sequence flanking the insertion site.

(Levis, 2018.8.30)

The PCR check of the insertion gave no band for the right flank and the expected sized product for the left flank.

(Levis, 2019.6.28)
Sequence Data
Flanking sequence
Inserted Element
Construct
TI{CRIMIC.GT14}
(Lee et al., 2018)
Size (Kb)
Component Allele(s)
Molecular map
Affected Gene(s)
Insertion may affect gene
Idh3a
(Levis, 2018.8.30)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

CR00027

(Lee et al., 2018, Levis, 2018.8.30)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
TI{CRIMIC.GT14}Idh3aCR00027
TI{CRIMIC.GT14}l(1)G0156CR00027
(Levis, 2018.8.30)
Name Synonyms
Secondary FlyBase IDs
    References (3)