FB2025_01 , released February 20, 2025
Insertion: Dmel\TI{CRIMIC.GT14}14-3-3ζCR99902
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General Information
Symbol
Dmel\TI{CRIMIC.GT14}14-3-3ζCR99902
Species
D. melanogaster
Name
FlyBase ID
FBti0200752
Feature type
insertion_site
Description
Inserted Element
TI{CRIMIC.GT14}
LINE ID

CR99902

Affected gene(s)
14-3-3ζ
Viability / fertility
Associated allele(s)
14-3-3ζCR99902
Stock availability
None publicly available
Genomic Location
Chromosomal Location
2R ( 46E6 )
Sequence Location
2R:10,105,495..10,105,517 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
Description
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:10,105,495..10,105,517 [+]
(Levis, 2018.8.30)
Orientation

+

(Levis, 2018.8.30)
Cytological location (computed by FlyBase)
46E6 (inferred by FlyBase from sequence location)
Cytological location (reported)
46E6_r6 (reported as inferred from sequence location)
(Levis, 2018.8.30)
Insertion into Natural transposon
Comments concerning location

The homology arms of the donor plasmid used in generating the TI{CRIMIC.GT14}14-3-3zetaCR99902 insertion were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion is predicted to be accompanied by a deletion of 22bp of genomic sequence flanking the insertion site.

(Levis, 2018.8.30)

The PCR check of the insertion gave the expected sized band for the left flank and a shorter than expected sized product for the right flank.

(Levis, 2019.6.28)
Sequence Data
Flanking sequence
Inserted Element
Construct
TI{CRIMIC.GT14}
(Lee et al., 2018)
Size (Kb)
Component Allele(s)
Molecular map
Affected Gene(s)
Insertion may affect gene
14-3-3ζ
(Levis, 2018.8.30)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

CR99902

(Lee et al., 2018, Levis, 2018.8.30)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
TI{CRIMIC.GT14}14-3-3zetaCR99902
(Levis, 2018.8.30)
TI{CRIMIC.GT14}14-3-3ζCR99902
Name Synonyms
Secondary FlyBase IDs
    References (3)