FlyBase Insertion Report: Dmel\TI{CRIMIC.GT14}unc-119[CR99921]

General Information

Symbol

Dmel\TI{CRIMIC.GT14}unc-119^CR99921

Species

D. melanogaster

Name

FlyBase ID

FBti0200758

Feature type

insertion_site

Description

Inserted Element

TI{CRIMIC.GT14}

LINE ID

CR99921

Affected gene(s)

CG1677, lncRNA:CR44357, unc-119

Viability / fertility

Associated allele(s)

CG1677^CR99921, lncRNA:CR44357^CR99921, unc-119^CR99921

Stock availability

None publicly available

Genomic Location

Chromosomal Location

X ( 7B1 )

Sequence Location

X:7,289,387..7,289,387 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

X:7,289,387..7,289,387 [-]

(Levis, 2019.12.5)

Orientation

(Levis, 2019.12.5)

Cytological location (computed by FlyBase)

7B1 (inferred by FlyBase from sequence location)

Cytological location (reported)

7B1_r6 (reported as inferred from sequence location)

(Levis, 2018.8.30)

Insertion into Natural transposon

Comments concerning location

The homology arms of the donor vector overlapped by 2 bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA. The PCR check of the insertion gave no product for either flank.

(Levis, 2019.6.28)

Sequence Data

Flanking sequence

Inserted Element

Construct

TI{CRIMIC.GT14}

(Lee et al., 2018)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene