A set of UAS-ORF transgenic constructs that are designed for use in a bimolecular fluorescence complementation (BiFC) assay. Each transgenic construct carries an ORF of interest tagged with a C-terminal N-m9Venus split fluorescent protein fragment and preceded by UASt regulatory sequences. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or the C-terminal tag sequence. All constructs also carry the w^+mC mini-white marker. Controlled expression of the ORFs can be effected by introduction of GAL4 drivers.