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General Information
Symbol
Dmel\TI{CRIMIC.TG4.2}wdeCR01355-TG4.2
Species
D. melanogaster
Name
FlyBase ID
FBti0206910
Feature type
Description
Inserted Element
Uses
Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
LINE ID

CR01355

Genomic Location
Chromosomal Location
2R ( 47C1 )
Sequence Location
2R:10,801,735..10,801,742 [-]
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
Description

A set of stocks that each contain a Trojan-GAL4 gene trap sequence in a coding intron, inserted via CRISPR/Cas9-induced homology directed repair. The inserted cassette contains a 'Trojan GAL4' gene trap element composed of a splice acceptor site followed by the T2A peptide, the GAL4 coding sequence and an SV40 polyadenylation signal. When inserted in a coding intron in the correct orientation, the cassette should result in truncation of the trapped gene product and expression of GAL4 under the control of the regulatory sequences of the trapped gene. Each line contains one of three versions of the cassette (TI{CRIMIC.TG4.0}, TI{CRIMIC.TG4.1} or TI{CRIMIC.TG4.2}), depending on the frame required to generate a gene trap. In addition, the presence of inverted attP sites flanking the inserted DNA allows for the entire cassette to be replaced with DNA from a compatible donor plasmid (where the sequence to be inserted is flanked by inverted attB sites) through recombination-mediated cassette exchange (RMCE) driven by the phiC31:int integrase.

Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:10,801,735..10,801,742 [-]
Cytological location (computed by FlyBase)
47C1 (inferred by FlyBase from sequence location)
Cytological location (reported)
47C1_r6 (reported as inferred from sequence location)
Insertion into Natural transposon
Comments concerning location

The homology arms of the donor plasmid used in generating the TI{CRIMIC.TG4.2}wdeCR01355-TG4.2 insertion were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion is predicted to be accompanied by a deletion of 7bp of genomic sequence flanking the insertion site.

Sequence Data
Flanking sequence
Inserted Element
Location-dependent role
Size (Kb)
Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene
Alleles and Phenotypes
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Linkouts
GDP-CRIMIC - A collection of T2A-GAL4 insertion lines made by CRISPR/Cas9-induced homology directed repair
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
TI{CRIMIC.TG4.2}wdeCR01355-TG4.2
Name Synonyms
Secondary FlyBase IDs
    References (1)