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General Information
Symbol
spCFP1-10
FlyBase ID
FBto0000068
Name
Description
Description

spCFP1-10 is a split fluorescent protein fragment that encodes 10 of the 11 strands of the GFP β-barrel structure. If the spCFP1-10 split fluorescent fragment is brought together with a complementary split fluorescent fragment (e.g. spGFP11) in vivo, a functional fluorescent protein may be reconstituted. spCFP1-10 was derived by mutagenesis of the spGFP1-10 split fluorescent fragment, introducing the mutations T65G, V68L, S72A, T203Y so that the reconstituted fluorescent protein is a Cerulean color variant (FBrf0230337). spCFP1-10 is designed for use in the 'GFP Reconstitution Across Synaptic Partners' (GRASP) technique (PMID:18255029), to study connectivity in the nervous system.

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Related experimental tools
Common gene of origin (62)
Tool
Uses
Transgenic Constructs
Tagged with the tool (1)
Transgenic construct(s)
Component allele
Reg. region
Encoded product / tool
Tagged with
Also carries
Stocks
Insertions ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
T:Avic\GFPspCFP1-10
spCFP1-10
Name Synonyms
Secondary FlyBase IDs
  • FBal0317192
References (3)