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General Information
Symbol
rtTA(M2-alt)
FlyBase ID
FBto0000174
Name
Description
Description

The rtTA(M2-alt) driver encodes a conditional transcriptional activator that contains a DNA-binding domain derived from the tetracycline repressor (tetR) gene of the transposon Tn10-encoded operon of Escherichia coli fused to the transcriptional activation domain from the Herpes simplex virus Type 1 VP16 gene. The tetR DNA-binding domain can bind specifically to the tetO DNA sequence and thus tetO and rtTA(M2-alt) form a conditional binary expression system that can be used to control the spatial and temporal expression of a gene of interest: a transgene or modified endogenous locus carrying the target gene of interest downstream of tetO sequences is combined with a second transgene or modified endogenous locus encoding the rtTA(M2-alt) driver. The tetR DNA-binding domain in the rtTA(M2-alt) driver has been mutated such that binding to tetO, and subsequent activation of transcription only occurs in the presence of tetracycline or related derivatives. Three additional modifications have been made in the tetR sequence of rtTA(M2-alt) compared to the rtTA driver: the 'M2' mutations shown to improve inducibility and specificity (PMID:10859354) have been introduced, a cryptic splicing site that created an intron between amino acids 8 and 144 has been removed and the codon usage has been optimized for mammalian cells (FBrf0138521).

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Transgenic Constructs
Encodes tool (2)
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Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
rtTA(M2-alt)
Name Synonyms
Secondary FlyBase IDs
    References (3)