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General Information
FlyBase ID
FLPo recombinase

FLPo is an artificial derivative of the recombinase encoded by the Saccharomyces cerevisiae 2μ plasmid. It has been optimized for mouse codon usage and contains the mutations P2S, G5D, L33S, Y108N and S294P relative to FLPG5 (PMID:17225864). This variant affects the activity of the recombinase rather than its target-site specificity. FLPo mediates recombination between a pair of specific target sites, with compatible target sites corresponding to the wild-type FRT site and some of its derivatives. The minimal FRT target site is composed of two 13bp inverted repeats flanking an 8bp asymmetric spacer sequence (PMID:3879971). Recombination occurs between a pair of target sites oriented in the same direction; the 13bp repeats each act as binding sites for the recombinase (PMID:3047402), while the asymmetric 8bp spacer is the site of DNA strand exchange and determines the orientation of the target site (PMID:3711092). In addition to being compatible with the wild-type FRT site, FLPo is expected to be compatible with any engineered FRT variant that contains a mutation only in the spacer region, but it is expected to be incompatible with FRT derivatives that contain mutations in their inverted repeats, since these latter mutations may affect the ability of the recombinase to bind the target site. The recombination event catalyzed by FLPo results in genetic modification, the nature of which is influenced by the relative orientation (direct or inverted), location and composition of the two target sites. The types of possible modification include deletion of DNA, generation of chromosomal rearrangements, integration of DNA into the genome, and replacement of genetic material with that from a donor plasmid using recombinase-mediated cassette exchange (RMCE) (reviewed in FBrf0231034).

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Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
FLPo recombinase
FLPo recombinase
Secondary FlyBase IDs
    References (9)