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General Information
Symbol
FLPm4
FlyBase ID
FBto0000314
Name
FLPm4 recombinase
Description
Description

FLPm4 is an engineered variant of the FLP recombinase encoded by the Saccharomyces cerevisiae 2μ plasmid, containing the mutations G5D, K82Y and V226A relative to FLPG5 (FBrf0213120, PMID:12547191). The target-site specificity of this variant is altered compared to wild-type FLP recombinase, such that FLPm4 is largely only compatible with the mFRT11 target site, a derivative of FRT that contains mutations in its inverted repeats (PMID:11917027, PMID:12547191). FLPm4 mediates recombination between a pair of mFRT11 sites. The recombination event results in genetic modification, the nature of which is influenced by the relative orientation (direct or inverted), location and composition of the two target sites. The types of possible modification include deletion of DNA, generation of chromosomal rearrangements and integration of DNA into the genome (reviewed in FBrf0231034).

Notes on Tool

FLPm4 shows some cross-reactivity towards the FRT site in an in vivo excision assay: recombination efficiency towards FRT is approximately one-quarter of that seen towards mFRT11.

External Crossreferences and Linkouts
Related experimental tools
Compatible tools (1)
Transgenic Constructs
Encodes tool (1)
Transgenic construct(s)
Component allele
Reg. region
Encoded product / tool
Tagged with
Also carries
Stocks
Insertions ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
FLPm4
Name Synonyms
FLPm4 recombinase
Secondary FlyBase IDs
    References (2)