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General Information
Symbol
FLPm5
FlyBase ID
FBto0000315
Name
FLPm5 recombinase
Description
Description

FLPm5 is an engineered variant of the FLP recombinase encoded by the Saccharomyces cerevisiae 2μ plasmid, containing the mutations G5D and R281Y relative to FLPG5 (FBrf0213120). The target-site specificity of this variant is altered compared to wild-type FLP recombinase, such that FLPm5 is highly specific for the mFRT71 target site, a derivative of FRT that contains mutations in its inverted repeats (PMID:12547191, FBrf0213120). FLPm5 mediates recombination between a pair of mFRT71 sites. The recombination event results in genetic modification, the nature of which is influenced by the relative orientation (direct or inverted), location and composition of the two target sites. The types of possible modification include deletion of DNA, generation of chromosomal rearrangements and integration of DNA into the genome (reviewed in FBrf0231034).

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Related experimental tools
Compatible tools (1)
Transgenic Constructs
Encodes tool (4)
Transgenic construct(s)
Component allele
Reg. region
Encoded product / tool
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Also carries
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Insertions ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
FLPm5
Name Synonyms
FLPm5 recombinase
Secondary FlyBase IDs
    References (4)