Open Close
General Information
Symbol
FLPm7
FlyBase ID
FBto0000317
Name
FLPm7 recombinase
Description
Description

FLPm7 is an engineered variant of the FLP recombinase encoded by the Saccharomyces cerevisiae 2μ plasmid, containing the mutations G5D, A36T, T50A, K82Y, G109N, T176A and R281V relative to FLPG5 (FBrf0213120). The target-site specificity of this variant is altered compared to wild-type FLP recombinase, such that FLPm7 is compatible with the mFRT11-71 target site, a derivative of FRT that contains mutations in its inverted repeats (FBrf0213120, PMID:12547191). FLPm7 mediates recombination between a pair of mFRT11-71 sites. The recombination event results in genetic modification, the nature of which is influenced by the relative orientation (direct or inverted), location and composition of the two target sites. The types of possible modification include deletion of DNA, generation of chromosomal rearrangements and integration of DNA into the genome (reviewed in FBrf0231034).

Notes on Tool

FLPm7 shows no recombinase activity towards the FRT or mFRT11 target sites in an in vivo excision assay. It shows some cross-reactivity with the mFRT71 site: 8.0% of cells carrying a mFRT71 cassette show an excision event compared to 67.2% of cells carrying a mFRT11-71 cassette.

External Crossreferences and Linkouts
Related experimental tools
Compatible tools (1)
Transgenic Constructs
Encodes tool (1)
Transgenic construct(s)
Component allele
Reg. region
Encoded product / tool
Tagged with
Also carries
Stocks
Insertions ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
FLPm7
Name Synonyms
FLPm7 recombinase
Secondary FlyBase IDs
    References (2)