FlyBase Experimental Tool Report: mKate2

General Information

Symbol

mKate2

FlyBase ID

FBto0000335

Name

mKate2 fluorescent protein

Description

Description

mKate2 is a monomeric fluorescent protein. It is an artificial derivative of the naturally occurring fluorescent protein encoded by the Entacmaea quadricolor eqFP578 gene, derived by mutagenesis of mKate. It is characterized by high brightness, excellent pH resistance and photostability and complete chromophore maturation. (PMID:19143658).

Uses

red fluorescent protein

External Crossreferences and Linkouts

FPbase - A database for users of fluorescent proteins.

DBBO8

Related experimental tools

Transgenic Constructs

Encodes tool (4)

Transgenic construct(s)

Component allele

Reg. region

Encoded product / tool

Tagged with

Also carries

Stocks

P{lexAop-UAS-mKate2.CAAX.K}

Equa\eqFP578^{mKate2.lexAop.UAS.Tag:CAAX(Unk)}

lexAop
UAS

mKate2: red fluorescent protein

Tag:CAAX(Unk): membrane protein localization tag

loxP: recombinase target site
lox2272: recombinase target site

P{lexAop-UAS-mKate2.NLS.K}

Equa\eqFP578^{mKate2.lexAop.UAS.Tag:NLS(Unk)}

lexAop
UAS

mKate2: red fluorescent protein

Tag:NLS(Unk): nuclear protein localization tag

loxP: recombinase target site
lox2272: recombinase target site

Equa\eqFP578^{mKate2.UAS.Tag:Palm(dgap43)}

UASt

mKate2: red fluorescent protein

Tag:Palm(dgap43): plasma membrane protein localization tag

P{UAS-brpD3-mKate2}

Equa\eqFP578^{mKate2.UAS.Tag:TS(brpS)}

UAS

mKate2: red fluorescent protein

Tag:TS(brpS): protein localization tag

Tagged with the tool (16)

Transgenic construct(s)

Component allele

Reg. region

Encoded product / tool

Tagged with

Also carries

Stocks

P{sK2MCA}

sqh

Moe

mKate2: red fluorescent protein

Eb1^{5xminiSOG.UAS.mKate2}

UASt

Eb1

mKate2: red fluorescent protein
miniSOG: reporter enzyme
chromophore-assisted light inactivation tag
light-regulated cell ablation tool

P{UASp-mKate2-Tm1.I}

Tm1^{UASp.I.mKate2}

UASp

Tm1

mKate2: red fluorescent protein

P{UAS-Syt1.5xminiSOG.mKate2}

Syt1^{5xminiSOG.UAS.mKate2}

UASt

Syt1

mKate2: red fluorescent protein
miniSOG: reporter enzyme
chromophore-assisted light inactivation tag
light-regulated cell ablation tool

P{-EGFP}

unc

unc

mKate2: red fluorescent protein

P{sqh.3xmKate2}

sqh

sqh

mKate2: red fluorescent protein

P{UASp-shot.ABD.mKate2}

shot^{ABD.UASp.mKate2}

UASp

shot

mKate2: red fluorescent protein

P{UASp-shot.ABD.V224D.mKate2}

shot^{ABD.V224D.UASp.mKate2}

UASp

shot

mKate2: red fluorescent protein

P{UAS-wkd.mKate2}

UASt

wkd

mKate2: red fluorescent protein

P{Ubi-spd-2.AA.mKate2}

spd-2^{AA.Ubi.mKate2}

spd-2

mKate2: red fluorescent protein

P{Ubi-spd-2.ALL.mKate2}

spd-2^{ALL.Ubi.mKate2}

spd-2

mKate2: red fluorescent protein

P{Ubi-spd-2.CONS.mKate2}

spd-2^{CONS.Ubi.mKate2}

spd-2

mKate2: red fluorescent protein

P{Ubi-spd-2.CT.mKate2}

spd-2^{CT.Ubi.mKate2}

spd-2

mKate2: red fluorescent protein

P{Ubi-spd-2.NT.mKate2}

spd-2^{NT.Ubi.mKate2}

spd-2

mKate2: red fluorescent protein

PBac{UASp-mKate2-AGO3.WT}

AGO3^UASp.mKate2

UASp

AGO3

mKate2: red fluorescent protein

PBac{UASp-mKate2-aub.WT}

aub^UASp.mKate2

UASp

aub

mKate2: red fluorescent protein

Insertions ( 16 )

Insertion

Encoded product / tool

Tagged with

Also carries

Stocks

Associated Alleles

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

T:Equa\eqFP578^mKate2

(FlyBase, 1992-)

T:Zzzz\eqFP578^mKate2

(FlyBase, 1992-)

mKate2

Name Synonyms

mKate2 fluorescent protein

Secondary FlyBase IDs

FBal0321178
FBal0320564
FBal0269037

References (17)

Report Sections