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General Information
FlyBase ID

The UASt entry in FlyBase represents an engineered regulatory region that includes UAS 'upstream activation' sequences plus a promoter fragment derived from Hsp70, where the Hsp70 fragment includes most of the Hsp70 5'UTR in addition to the TATA box. The UASt regulatory region was originally constructed as part of the pP{UAST} cloning vector (FBrf0064375). The UAS regulatory sequence present in UASt is specifically bound by the DNA-binding domain of the Saccharomyces cerevisiae GAL4 gene ( SGDID:S000006169 ) (PMID:3886158). This property can be utilized to form a binary expression system to control the spatial and temporal expression of a gene of interest: a transgene or modified endogenous locus in which the target gene of interest is downstream of the UASt regulatory region can be combined with a transgene or modified endogenous locus encoding any 'driver' that includes the GAL4 DNA-binding domain plus a transcriptional activation sequence (FBrf0064375, also reviewed in FBrf0233764, FBrf0216478). Transgenes that contain the UASt regulatory region are expressed in somatic tissues in the presence of an appropriate driver, but are typically either not expressed or are expressed at lower levels in the female germline during oogenesis. The low level of expression in the female germline is due to silencing by Hsp70-directed piRNAs produced in this tissue; these piRNAs are able to target sequences within the Hsp70 5'UTR that is present in the UASt regulatory sequence (FBrf0239059).

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Transgenic Constructs
Carrying the tool (4)
Has tool as regulatory region (48153)
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Insertions ( 12,391 )