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General Information
Symbol
UASp
FlyBase ID
FBto0000343
Name
Description
Description
The UASp entry in FlyBase represents an engineered regulatory region that includes UAS 'upstream activation' sequences plus a promoter fragment derived from the P-element transposase gene (P\T), where the P\T fragment includes the 5'UTR in addition to the promoter. The UASp regulatory region was originally constructed as part of the pP{UASp} cloning vector (FBrf0105917). The UAS regulatory sequence present in UASp is specifically bound by the DNA-binding domain of the Saccharomyces cerevisiae GAL4 gene ( SGDID:S000006169 ) (PMID:3886158). This property can be utilized to form a binary expression system to control the spatial and temporal expression of a gene of interest: a transgene or modified endogenous locus in which the target gene of interest is downstream of the UASp regulatory region can be combined with a transgene or modified endogenous locus encoding any 'driver' that includes the GAL4 DNA-binding domain plus a transcriptional activation sequence (FBrf0064375, also reviewed in FBrf0233764, FBrf0216478). Transgenes that contain the UASp regulatory region are expressed in the female germline (FBrf0105917), but are often less well expressed in somatic tissues.
External Crossreferences and Linkouts
Related experimental tools
Transgenic Constructs
Has tool as regulatory region (2012)
Transgenic construct(s)
Component allele
Reg. region
Encoded product / tool
Tagged with
Also carries
Stocks
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Insertions ( 4,061 )
Insertion
Encoded product / tool
Tagged with
Also carries
Stocks
Associated Alleles
List limited to the first 200 records. Use the HitList export button in the section header to view all records.
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
UASp
Name Synonyms
Secondary FlyBase IDs
    References (701)