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General Information
Symbol
loxP
FlyBase ID
FBto0000349
Name
locus of crossing over, P1
Description
Description
The loxP entry in FlyBase represents the wild-type target site for the cre recombinase encoded by the bacteriophage P1 genome. The 34bp loxP site (5'-ATAACTTCGTATAatgtatgcTATACGAAGTTAT-3') is composed of two 13bp inverted repeats flanking an 8bp asymmetric spacer sequence (PMID:6954485). Recombination occurs between a pair of target sites oriented in the same direction; the 13bp repeats each act as binding sites for the recombinase, while the asymmetric 8bp spacer is the site of DNA strand exchange and determines the orientation of the target site (PMID:3856690). The recombination event catalyzed by the cre recombinase results in genetic modification, the nature of which is influenced by the relative orientation (direct or inverted), location and composition of the two target sites. The types of possible modification include deletion of DNA and generation of chromosomal rearrangements (reviewed in FBrf0231034).
Notes on Tool
PMID:17972876 demonstrates that loxP is incompatible with lox2272 in vivo (HEK cells).
External Crossreferences and Linkouts
Related experimental tools
Transgenic Constructs
Carrying the tool (17113)
Transgenic construct(s)
Component allele
Reg. region
Encoded product / tool
Tagged with
Also carries
Stocks
List limited to the first 200 records. Use the HitList export button in the section header to view all records.
Insertions ( 4,286 )
Insertion
Encoded product / tool
Tagged with
Also carries
Stocks
Associated Alleles
List limited to the first 200 records. Use the HitList export button in the section header to view all records.
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
loxP
Name Synonyms
locus of crossing over, P1
loxP recombination site
Secondary FlyBase IDs
  • FBgn0016995
References (286)