Recombinant construct P{CaSpeR}

General Information
Symbol	P{CaSpeR}	FlyBase ID	FBtp0000155
Feature type	transgenic_transposon

Size	5.016	Expression data
Associated insertions	65 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Pirrotta, 1988)
Uses	CV term Qualifiers & info Reference carrier transposon P-element (Pirrotta, 1988) genomic insertion screen
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent X81644
Segments & Size
Total Size	5.016
Left end	P5\' (Pirrotta, 1988)
Right end	P3\' (Pirrotta, 1988)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + M13mp10mcs 3 + Car1_P3'(H3,P3')
Reference	(Pirrotta, 1988)
Features
	CV term Qualifiers & info Reference partial rescue (Pirrotta, 1988) selectable marker (Pirrotta, 1988, Sved et al., 1990, Baldarelli and Lengyel, 1990, Thummel and Pirrotta, 1991, Sved et al., 1991, Pereira et al., 1992, Roseman et al., 1993, Prokchorova et al., 1994, Qian and Pirrotta, 1995, Svoboda et al., 1995, Gray et al., 1996, Preston et al., 1996, Preston and Engels, 1996, Tanaka et al., 1997, Zagrodzinska and Kloc, 1998)
Component Alleles
Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Lloyd et al., 2002, Roseman et al., 1993, Prokchorova et al., 1994, Svoboda et al., 1995, Qian and Pirrotta, 1995, Tanaka et al., 1997, Baldarelli and Lengyel, 1990, Pereira et al., 1992, Thummel and Pirrotta, 1991, Sved et al., 1991, Sved et al., 1990, Gray et al., 1996, Preston and Engels, 1996, Preston et al., 1996, Zagrodzinska and Kloc, 1998)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{CaSpeR} (Pirrotta, 1988)
descendant(s)	P{CaSpeR-C47.1} (Engels et al., 1990)
Comments
	construct_comment: Good map of pP{CaSpeR} shown in Figure 5 in FBrf0068634. (Petersen et al., 1994) construct_comment: One of a series of transposons based upon P{CaSpeR} and differing in cloning sites (cloning sites listed in construct reports). (Thummel and Pirrotta, 1991) report comment: Compiled sequence initiates at different position and is in opposite orientation relative to sequence entry listed. See also report for component segment w^+mC4.1(Sp): sequence discrepancies. (Pirrotta, 1988)
Stocks Listed in FlyBase ( 8 )
Bloomington	11304 w^* P{CaSpeR}C19.1a P{CaSpeR}C19.1b 11323 P{CaSpeR}cx35.1/C(1)DX, y¹ f¹ 11326 P{CaSpeR}cx131.6/C(1)DX, y¹ w¹ f¹ 11328 P{CaSpeR}cx134.16/C(1)DX, y¹ w¹ f¹ 8111 Df(2R)50C-102, P{CaSpeR}Cp1^50C-102 bw¹/CyO, amos^Roi-1 bw¹ 8113 Df(2R)50C-36, al¹ b¹ cn¹ P{CaSpeR}Cp1^50C-36/CyO, amos^Roi-1 bw¹ 8114 Df(2R)50C-38, al¹ b¹ cn¹ P{CaSpeR}Cp1^50C-38/CyO, amos^Roi-1 bw¹ 8112 Df(2R)50C-129, al¹ b¹ cn¹ P{CaSpeR}Cp1^50C-129/CyO, amos^Roi-1 bw¹
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	CaSpeR (Pirrotta, 1988) P{CaSpeR-1} (FlyBase, 1996-) P{CaSpeR} (FlyBase, 1996-, Engels, 1990.8.1, Pirrotta, 1994.9.14) P{w^+mC=CaSpeR} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 21 )

Generate a list of	All references relating to this transgenic transposon ( 21 )

List References by type	Research paper ( 18 ) Review ( 1 ) Personal communication to FlyBase ( 1 ) DNA/RNA sequence record ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...
Recent reviews (0)
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