Recombinant construct P{lAwB}

General Information
Symbol	P{lAwB}	FlyBase ID	FBtp0000158
Feature type	transgenic_transposon

Size	15.653	Expression data	lacZ reporter
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role	lacZ enhancer trap (Wilson et al., 1989)
Description	CV term Qualifiers & info Reference transposon P-element (Wilson et al., 1989)
Uses	CV term Qualifiers & info Reference enhancer trap P-element (Wilson et al., 1989)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	15.653
Left end	P5\' (Wilson et al., 1989)
Right end	P3\' (Wilson et al., 1989)

Segments	Number Orientation Symbol 1 + P\T::lacZ(P5',R1)b 2 + car2mcs 3 + 001008 4 - Adh⁺3.2(Xb) 5 + 001009 6 + w^+mW4.6(Ha3,R1) 7 + 001006 8 - pBLM-mcs(K,Sc1) 9 + 001007 10 + Car1_P3'(H3,P3')
Reference	(Wilson et al., 1989)
Features
	CV term Qualifiers & info Reference bacterial selection (Wilson et al., 1989) chimeric gene (Klemenz et al., 1987) chimeric gene (Wilson et al., 1989) complete rescue (Wilson et al., 1989) enhancer trap (Wilson et al., 1989) partial rescue (Posakony et al., 1985) partial rescue (Klemenz et al., 1987) reporter gene (Wilson et al., 1989) selectable marker (Wilson et al., 1989, Posakony et al., 1985) selectable marker (Klemenz et al., 1987, Wilson et al., 1989)
Component Alleles
Allele	Adh^+t3.2
Reference(s)	(Wilson et al., 1989)
Molecular data	3.2kb XbaI genomic fragment containing the Adh gene. (Kirkpatrick et al., 1994) 3.2kb XbaI fragment containing Adh. (Ayer and Benyajati, 1992) Construct: Contained within 3.2kb genomic XbaI fragment. (Kirkpatrick and Martin, 1992) 3.2kb XbaI fragment that contains the Adh⁺ function. (Sass, 1990) A 3.2kb XbaI fragment that contains Adh⁺ function. (Wilson et al., 1989) A 3.2kb XbaI fragment that carries the Adh^F allele. (Pirrotta, 1988) 3.2kb Adh XbaI fragment containing 663 nucleotides of the 5' region. (Martin et al., 1986) 3.2kb XbaI Adh fragment which has 0.66kb of 5' flanking sequences and 0.64kb of 3' flanking sequences, all of the cis-acting DNA sequences necessary for the major tissue specificities of Adh expression. (Posakony et al., 1985)
Phenotypic class
Phenotype manifest in
Other information

Allele	Ecol\lacZ^P\T.A92a
Reference(s)	(Wilson et al., 1989)
Molecular data	Construct: In frame translational fusion of P\T to Ecol\lacZ, in the second exon of the P element. The fusion includes the P element promoter. The P-element 5' end is from pUChsneo. (Wilson et al., 1989)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mW.hs
Reference(s)	(Klemenz et al., 1987, Wilson et al., 1989)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage epidermis (Perrimon et al., 1991) organism \| 10-100% egg length (Perrimon et al., 1991) • nucleus (Perrimon et al., 1991) extended germ band stage dorsal ectoderm \| segmentally repeated (Bellen et al., 1989) embryonic stage 9 -- 12 procephalic neurogenic region (Bellen et al., 1989)
Additional Information
Statement Reference Basal pattern of stripes, restricted to epidermal clusters of cells. Expression localized to nucleus. (Perrimon et al., 1991) Expression pattern dependent upon position of insertion in genome. Basal pattern observed in approximately 30% of transformants: in extended germ band embryos, weak expression in dorsal epidermis, stronger in alternate segments. (Bellen et al., 1989)

Marker for
Reflects expression of	P\T (Bellen et al., 1989)
Progenitors & Descendants
progenitor(s)	pP{lAwB} (Wilson et al., 1989)
descendant(s)
Comments
	construct_comment: One of a series of constructs containing the lacZ enhancer trap lacZ^P\T.A92a, differing in selectable markers. (Wilson et al., 1989)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[lAwB] (Wilson et al., 1989) P{lacZ^P\T.A92a Adh^+t3.2 w^+mW.hs ampR ori=lAwB} (FlyBase, 1996-) P{lAwB} (FlyBase, 1996-, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 2 )
Research paper	Wilson et al., 1989, Genes Dev. 3(9): 1301--1313 P-element-mediated enhancer detection: an efficient method for isolating and characterizing developmentally regulated genes in Drosophila. [FBrf0049802]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]