Recombinant construct P{CaSpeR-4}

General Information
Symbol	P{CaSpeR-4}	FlyBase ID	FBtp0000163
Feature type	transgenic_transposon

Size	5.103	Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Thummel and Pirrotta, 1991)
Uses	CV term Qualifiers & info Reference carrier transposon P-element (Thummel and Pirrotta, 1991)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent X81645
Segments & Size
Total Size	5.103
Left end	P5\' (Thummel and Pirrotta, 1991)
Right end	P3\' (Thummel and Pirrotta, 1991)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + pW8mcs+H3 3 + Car1_P3'(H3,P3')
Reference	(Thummel and Pirrotta, 1991)
Features
	CV term Qualifiers & info Reference partial rescue (Pirrotta, 1988) selectable marker (Pirrotta, 1988, Qian et al., 1991, Bhadra et al., 1997, Cuvier et al., 1998)
Component Alleles
Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Qian and Pirrotta, 1995, Thummel and Pirrotta, 1991, Qian et al., 1991, Cuvier et al., 1998, Bhadra et al., 1997)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{CaSpeR-4} (Thummel and Pirrotta, 1991)
descendant(s)	P{hsILMiT} (Metaxakis et al., 2005)
Comments
	construct_comment: Good map of pP{CaSpeR} shown in Figure 5 in FBrf0068634. (Petersen et al., 1994) construct_comment: One of a series of transposons based upon P{CaSpeR} and differing in cloning sites (cloning sites listed in construct reports). (Thummel and Pirrotta, 1991) report comment: Compiled sequence initiates at different position and is in opposite orientation relative to sequence entry listed. See also report for component segment w^+mC4.1(Sp): sequence discrepancies. (Thummel and Pirrotta, 1991)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	CaSpeR_4 (Thummel and Pirrotta, 1991) P{CaSpeR-4} (FlyBase, 1996-, Pirrotta, 1994.9.14) P{w⁺mC</up>=CaSpeR-4} (FlyBase, 1996-) P{w^+mC=CaSpeR-4} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 10 )
Research paper	Metaxakis et al., 2005, Genetics 171(2): 571--581 Minos as a genetic and genomic tool in Drosophila melanogaster. [FBrf0190750] Cuvier et al., 1998, Mol. Cell. Biol. 18(12): 7478--7486 Identification of a class of chromatin boundary elements. [FBrf0105485] Bhadra et al., 1997, Mol. Gen. Genet. 254(6): 621--634 A trans-acting modifier causing extensive overexpression of genes in Drosophila melanogaster. [FBrf0094159] Qian and Pirrotta, 1995, Genetics 139(2): 733--744 Dosage compensation of the Drosophila white gene requires both the X chromosome environment and multiple intragenic elements. [FBrf0080335] Petersen et al., 1994, Genetics 136(1): 173--182 forked proteins are components of fiber bundles present in developing bristles of Drosophila melanogaster. [FBrf0068634] Thummel and Pirrotta, 1992, D. I. S. 71: 150 Technical notes: new pCasper P-element vectors. [FBrf0082699] Qian et al., 1991, EMBO J. 10: 1415--1425 The bx region enhancer, a distant cis-control element of the Drosophila Ubx gene and its regulation by hunchback and other segmentation genes. [FBrf0053871] Thummel and Pirrotta, 1991, D. I. N. 2: Technical Notes: New pCaSpeR P-element vectors. [FBrf0053508]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]
DNA/RNA sequence record	Pirrotta, 1994.9.14, GenBank/EMBL/DDBJ: X81645 D.melanogaster P element CaSpeR-4 gene. [FBrf0172391]