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General Information
Symbol
P{ftz-lacC}
FlyBase ID
FBtp0000176
Feature type
transgenic_transposon
Component Allele(s)
Also Known As
ftz-lacZ
Size
18.592
Expression Data
lacZ reporter
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Product class / Tool use(s)
Marker(s)
Component allele Ecol\lacZftz.PC
Product class / Tool use(s)
Encoded product / tool
Description

A full length ftz promoter fragment which extends from -6.1kb (relative to the translation start site) to the second codon is fused in frame to lacZ.

Approximately 6kb of ftz 5' flanking sequence, including the zebra, neurogenic and upstream elements drives lacZ expression.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size

18.592

Left end
Right end
 
Features
CV term
Qualifiers and info
Reference
chimeric
gene
reporter
gene
Component Alleles
Molecular data

Approximately 6kb of ftz 5' flanking sequence, including the zebra, neurogenic and upstream elements drives lacZ expression.

A full length ftz promoter fragment which extends from -6.1kb (relative to the translation start site) to the second codon is fused in frame to lacZ.

Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
radioisotope in situ
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

ftz is expressed in alternating parasegments in the germ band retracted embryo between parasgements 2 and 12.

In germ-band extended embryos, strong expression in the mesoderm and ectoderm, in even parasegments. In later embryos, expression is observed in the CNS. Expression pattern characteristic of that observed for ftz.

In embryos, peak accumulation is from 4.5-10.5 hours after egg-laying.

In gastrulating embryos, the first stripe is posterior to the cephalic furrow. Seven stripes observed; most posterior stripe thicker and occasionally resolves as two stripes. During mid-embryogenesis expression also observed in pairs of large cells; assumed to be neuroblasts. The most conspicuous of these pairs flank the ventral midline, one pair per segment. Observed stripes in epidermis correspond to parasegments, not segments. Expression throughout the ventral nerve cord (not in pair-rule pattern).

 
Marker for
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments

construct_comment: Defined 3 regulatory regions: the zebra element, the neurogenic element, and the upstream element.

report comment: Contains the ftz "zebra element", the "neurogenic element" and the "upstream element" enhancers.

construct_comment: One of a series of lacZ reporter constructs designed to characterize 5\' regulatory elements of ftz.

Stocks (60)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (13)
References (54)