Recombinant construct P{lacW}

General Information
Symbol	P{lacW}	FlyBase ID	FBtp0000204
Feature type	transgenic_transposon

Size	10.691	Expression data	lacZ reporter
Associated insertions	7102 available
Molecular map
Recent Updates
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FB2011_10	Stocks y[1] w[*]; P{w[+mC]=lacW}CG6194[P0997] References Malartre et al., 2010, J. Neurosci. 30(6): 2257--2267
FB2012_01	Insertions P{lacW}P116 P{lacW}hipk[S134313] P{lacW}P14 P{lacW}P901 P{lacW}P23 P{lacW}P9 P{lacW}P42 P{lacW}P545 P{lacW}P3 P{lacW}P4 P{lacW}P132 P{lacW}P472 P{lacW}Ent2[P124] P{lacW}P171[P171] P{lacW}P211 P{lacW}P21 P{lacW}P10 P{lacW}P47 P{lacW}P30 P{lacW}P45 P{lacW}P80 P{lacW}P12 P{lacW}l(3)S097074[S097074a] P{lacW}CK4A P{lacW}P100-I P{lacW}Gug[S146907] P{lacW}P142 P{lacW}P71 P{lacW}P95[P95] P{lacW}P124b P{lacW}P31 P{lacW}P34 P{lacW}P97 P{lacW}P25 P{lacW}P471 P{lacW}P11 P{lacW}P141 References Kamyshev et al., 2000, Symp. Molecular and genetic bases of adaptive behavior: 307--314 Link et al., 2007, J. Cell Biol. 178(4): 567--574 Williams et al., 2007, J. Cell Sci. 120(20): 3522--3533 Alleles P171[P171] Ent2[P124] hipk[S134313] Gug[S146907] Ecol\lacZ[trio-S137203] CoVIII[S055418] P95[P95] bel[S097074]
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Description	CV term Qualifiers & info Reference transposon P-element (Bier, 1995.10.13, Bier et al., 1989, Peter et al., 2002, Parks et al., 2004, Bellen et al., 2004)
Uses	CV term Qualifiers & info Reference enhancer trap P-element (Bier et al., 1989) genomic insertion screen lethal (Schaefer et al., 1999.3.29, Roch et al., 1998, Schaefer et al., 1999.6.15, Schaefer et al., 1999.8.12, Schaefer et al., 1999.12.10, Schaefer et al., 1999.12.14, Schaefer et al., 1999.12.2, BDGP Project Members, 1994-1999, Deak et al., 1997, Salzberg et al., 1997)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	10.691
Left end	P5\' (Bier, 1995.10.13)
Right end	P3\' (Bier, 1995.10.13)

Segments	Number Orientation Symbol 1 + P\T::lacZ(P5',Bg2) 2 + 001094 3 + w^+mC4.1(Sp) 4 + 001095 5 - pBR322ori/amp(-Pst) 6 + 001096 7 + 6.1_P3'(X1,P3')
Reference	(Bier et al., 1989)
Features
	CV term Qualifiers & info Reference bacterial selection (Bier et al., 1989) chimeric gene (Bier, 1995.10.13) enhancer trap (Bier, 1995.10.13) partial rescue (Pirrotta, 1988) reporter gene (Bier et al., 1989) selectable marker (Pirrotta, 1988, Bier et al., 1989, Dorer and Henikoff, 1994, Huang and Smith, 1995, Deak et al., 1995, Sun et al., 1995, Brodsky and Steller, 1996, True et al., 1996, True et al., 1996, Bhadra et al., 1997, Martin-Morris et al., 1997, Dorer and Henikoff, 1997, Fanti et al., 1998, Csink et al., 2002)
Component Alleles
Allele	Ecol\lacZ^P\T.W
Reference(s)	(Bier, 1995.10.13, Bier et al., 1989, Ronsseray et al., 2001, Huang and Smith, 1995, Wines and Henikoff, 1992)
Molecular data	Ecol\lacZ gene expression under the control of the P-element transposase promoter gene region. (Wines and Henikoff, 1992) Construct: In frame translational fusion of P\T to Ecol\lacZ, including the weak P\T transposase promoter, in approximately the same position as for the Ecol\lacZ^P\T.A92 allele reported in FBrf0047244. (Bier et al., 1989)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Csink et al., 2002, Perrin et al., 2003, Dorer and Henikoff, 1994, Huang and Smith, 1995, Kuhfittig et al., 2001, Dorer and Henikoff, 1997, Martin-Morris et al., 1997, Bier et al., 1989, Fanti et al., 1998, True et al., 1996, True et al., 1996, Brodsky and Steller, 1996, Deak et al., 1995, Sun et al., 1995, Bhadra et al., 1997)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference Observed expression always nuclear, independent of insertion site. (Bier et al., 1989) In embryos, pattern of expression dependent upon position of insertion. Observed expression always nuclear, independent of insertion site. (Bier et al., 1989)

Assay mode
Marker for
Reflects expression of	P\T (Bier et al., 1989)
Progenitors & Descendants
progenitor(s)	pP{lacW} (Bier et al., 1989)
descendant(s)	P{3'lacW.2Bab-10} (Gonzy-Treboul et al., 1995) P{3'lacW.3HP} (Ring et al., 2000) P{3'lacW.5R5.24} (Szuplewski et al., 2003) P{3'lacW} (FlyBase, 1992-) P{5'lacW.5R1.5} (Szuplewski et al., 2003) P{5'lacW.5R7.2} (Szuplewski et al., 2003) P{5'lacW} (FlyBase, 1992-) P{?'lacW} (FlyBase, 1992-) P{?lacW} (FlyBase, 1992-) P{lacW.ph+2} (Fauvarque et al., 1995) P{lacW.ph+3} (Fauvarque et al., 1995) P{lacW-Δ2.R} (Ruhf et al., 2001) P{lacW-Δ3.R} (Ruhf et al., 2001) P{lacW-Δ4} (Roman et al., 2000) P{lacW-Δ5M} (Rodrigues et al., 1995) P{lacW-Δ6.R} (Ruhf et al., 2001) P{lacW-Δ7.R} (Ruhf et al., 2001) P{lacW-Δ7id} (Beck et al., 2004) P{lacW-Δ9.R} (Ruhf et al., 2001) P{lacW-Δ10.R} (Ruhf et al., 2001) P{lacW-Δ11.R} (Ruhf et al., 2001) P{lacW-Δ12.M} (Meyer et al., 1998) P{lacW-Δ12.R} (Ruhf et al., 2001) P{lacW-Δ12} (Rodrigues et al., 1995) P{lacW-Δ13.R} (Ruhf et al., 2001) P{lacW-Δ15.R} (Ruhf et al., 2001) P{lacW-Δ16.R} (Ruhf et al., 2001) P{lacW-Δ17.R} (Ruhf et al., 2001) P{lacW-Δ18.R} (Ruhf et al., 2001) P{lacW-Δ80BL} (Gonzy et al., 2002) P{lacW-Δ.5HP} (Ring et al., 2000) P{lacW-Δexc2} (Taillebourg and Dura, 2000) P{lacW-Δexc8} (Taillebourg and Dura, 2000) P{lacW-Δix1} (Jackson and Berg, 2002) P{lacW-Δix2} (Jackson and Berg, 2002) P{lacW-Δix4} (Jackson and Berg, 2002) P{lacW-ΔR18} (Hartenstein et al., 1997) P{lacW-ΔR27} (Hartenstein et al., 1997) P{lacW-ΔR33} (Hartenstein et al., 1997) P{lacW-ΔR38} (Hartenstein et al., 1997) P{lacW-ΔS089302rv1} (Kuhfittig et al., 2001) P{lacW-ΔS089302rv6} (Kuhfittig et al., 2001) P{lacW-ΔS089302rv7} (Kuhfittig et al., 2001) P{lacW-ΔS089302rv9} (Kuhfittig et al., 2001)
Comments
	construct_comment: Restriction sites useful for plasmid rescue: (right) EcoRI, SacII; (left) XbaI, BglII, PstI, BamHI. (Bier et al., 1989)
Stocks Listed in FlyBase ( 2606 )
Bloomington	4358 P{lacW}439 6317 w^; P{lacW}J29 6970 w^; P{lacW}M84 9302 w^; P{lacW}rin¹ 27896 w^; P{lacW}mth¹ 6315 y¹ w^; P{lacW}M64 6316 y¹ w^; P{lacW}G38 6319 y¹ w^; P{lacW}K61 6321 y¹ w^; P{lacW}J45 845 w¹¹¹⁸ P{lacW}sn^AL2
Kyoto	109637 P{lacW}Fili^lacZ 103372 w^* ; P{lacW}k11038 / CyO
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW} (FlyBase, 1996-, Sun et al., 1995, BDGP Project Members, 1994-1999, Roch et al., 1998, Schaefer et al., 1999.12.10, Schaefer et al., 1999.12.14, Bellen et al., 2004, Schaefer et al., 1999.6.15, Hartenstein and Jan, 1992, Gonczy et al., 1992, Schaefer et al., 1999.12.2, Torok et al., 1993, Szeged Stock Center, 1998-, Parks et al., 2004, Deak et al., 1997, Salzberg et al., 1997, Wasserman, 1990.8.1, Peter et al., 2002, Schaefer et al., 1999.8.12, Schaefer et al., 1999.3.29) P{lacZ^P\T.W w^+mC ampR ori=lacW} (FlyBase, 1996-) P-lacW (Bier et al., 1989)
Secondary FlyBase IDs

References ( 127 )

Generate a list of	All references relating to this transgenic transposon ( 127 )

List References by type	Research paper ( 101 ) Supplementary material ( 5 ) Review ( 1 ) Personal communication to FlyBase ( 12 ) Abstract ( 2 ) FlyBase analysis ( 4 ) Computer file ( 2 )
Recent research papers ( 5 )
	Poulton et al., 2011, Development 138(9): 1737--1745 The microRNA pathway regulates the temporal pattern of Notch signaling in Drosophila follicle cells. [FBrf0213494] Chakrabarti et al., 2010, Neuron 66(6): 835--847 Mitochondrial Dysfunction in NnaD Mutant Flies and Purkinje Cell Degeneration Mice Reveals a Role for Nna Proteins in Neuronal Bioenergetics. [FBrf0211249] Malartre et al., 2010, J. Neurosci. 30(6): 2257--2267 The Guanine Exchange Factor vav Controls Axon Growth and Guidance during Drosophila Development. [FBrf0209937] Silies and Klämbt, 2010, Nat. Neurosci. 13(11): 1357--1364 APC/C(Fzr/Cdh1)-dependent regulation of cell adhesion controls glial migration in the Drosophila PNS. [FBrf0212144] Taniue et al., 2010, Development 137(10): 1755--1764 Sunspot, a link between Wingless signaling and endoreplication in Drosophila. [FBrf0210642] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...