Recombinant construct P{PZ}

General Information
Symbol	P{PZ}	FlyBase ID	FBtp0000210
Feature type	transgenic_transposon

Size	14.545	Expression data
Associated insertions	1743 available
Molecular map
Recent Updates
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FB2012_01	References Belgacem and Martin, 2007, PLoS ONE 2: Andersen et al., 2010, Mol. Cell. Biol. 30(19): 4744--4755
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Description	CV term Qualifiers & info Reference transposon P-element (Mlodzik and Hiromi, 1992, Parks et al., 2004, Bellen et al., 2004)
Uses	CV term Qualifiers & info Reference enhancer trap P-element (Mlodzik and Hiromi, 1992) genomic insertion screen lethal (BDGP Project Members, 1994-1999) male sterile (Zhang and Stankiewicz, 1998, Castrillon et al., 1993)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	14.545
Left end	P5\' (Mlodzik and Hiromi, 1992)
Right end	P3\' (Mlodzik and Hiromi, 1992)

Segments	Number Orientation Symbol 1 + P\T::lacZ(P5',u) 2 + 001115a 3 + 001115b 4 - neo^r1.3(H3,Sm) 5 + 001114 6 + PSJ1_191 7 + pHSS6_ori 8 + 001116 9 + Car20_P3'(H3,P3')
Reference	(Mlodzik and Hiromi, 1992)
Features
	CV term Qualifiers & info Reference bacterial selection (Mlodzik and Hiromi, 1992) chimeric gene (Mlodzik and Hiromi, 1992) complete rescue (Spradling and Rubin, 1983) enhancer trap (Mlodzik and Hiromi, 1992) reporter gene (Mlodzik and Hiromi, 1992) selectable marker (Rubin and Spradling, 1983, Mlodzik and Hiromi, 1992)
Component Alleles
Allele	Ecol\lacZ^P\T.PZ
Reference(s)	(Mlodzik and Hiromi, 1992, Zhang et al., 2000)
Molecular data	Construct: Ecol\lacZ gene expression is driven by the P\T promoter and transposase sequences upto the 125th codon of exon 2. (Mlodzik and Hiromi, 1992)
Phenotypic class
Phenotype manifest in
Other information

Allele	l(3)87Df^7.2
Reference(s)	(Horowitz and Berg, 1995)
Molecular data	Construct: Portion of l(3)87Df containing an intron of 70 nucleotides, the splice acceptor site and the remaining coding sequence and transcription termination sequences. (Horowitz and Berg, 1995)
Phenotypic class
Phenotype manifest in
Other information

Allele	ry^+t7.2
Reference(s)	(Rubin and Spradling, 1983, Mlodzik and Hiromi, 1992)
Molecular data	Construct: 7.2kb HindIII fragment that contains ry⁺ function. (Rubin and Spradling, 1983)
Phenotypic class	wild-type (Rubin and Spradling, 1983)
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{PZ} (Mlodzik and Hiromi, 1992)
descendant(s)	P{3'PZ} (FlyBase, 1992-) P{5'3'} (Frolov and Birchler, 1998) P{5'PZ.64} (Barges et al., 2000) P{5'PZ} (FlyBase, 1992-) P{?'PZ} (FlyBase, 1992-) P{?PZ} (FlyBase, 1992-) P{PZ-Δ.a2} (Gonzalez-Gaitan and Jackle, 1997) P{PZ-Δ.p51} (Simin et al., 1998) P{PZ-Δ.p62} (Simin et al., 1998) P{PZ-Δ.p88} (Simin et al., 1998) P{PZ-Δ.s2} (Schulze and Bellen, 1996) P{PZ-Δ.s4} (Schulze and Bellen, 1996) P{PZ-Δ.s17} (Schulze and Bellen, 1996) P{PZ-Δ.s118} (Schulze and Bellen, 1996) P{PZ-Δ.s177} (Schulze and Bellen, 1996) P{PZ-Δ.s287} (Schulze and Bellen, 1996) P{PZ-Δ.s294} (Schulze and Bellen, 1996) P{PZ-ΔE38} (Nakagoshi et al., 1998) P{PZ-Δix12a} (Schnorr and Berg, 1996) P{PZ-Δix13b} (Schnorr and Berg, 1996) P{PZ-ΔrR3-4} (Rose et al., 1997) P{PZ-ΔrR5} (Rose et al., 1997) P{PZ-ΔrR7} (Rose et al., 1997)
Comments
	sequence comment: Included as non-attributed correction. (Benes, 1997.7.25) mapping_comment: Included as non-attributed correction. (Benes, 1997.7.25) report comment: Thanks to Brian Calvi and Vladimir Benes for assistance in assembling compiled sequence. Included as non-attributed correction. (Benes, 1997.7.25) mapping_comment: (Mlodzik and Hiromi, 1992) report comment: (Mlodzik and Hiromi, 1992) construct_comment: Restriction site useful for plasmid rescue: XbaI. (Mlodzik and Hiromi, 1992) sequence comment: (Mlodzik and Hiromi, 1992)
Stocks Listed in FlyBase ( 563 )
Bloomington	4159 ry⁵⁰⁶ P{PZ}900 5374 P{PZ}ap^rK568/CyO 5530 ry⁵⁰⁶ P{PZ}hh^P30 25079 P{PZ}dpr¹; ry⁵⁰⁶ 9093 P{PZ}Gli^rL82; ry⁵⁰⁶ 684 ry⁵⁰⁶ P{PZ}fru³/MKRS 11611 ry⁵⁰⁶ P{PZ}MI02⁰³⁸⁴⁷ 11743 ry⁵⁰⁶ P{PZ}btsz¹⁰⁴¹⁸ 12047 P{PZ}dac^P/CyO; ry⁵⁰⁶ 12171 cn¹ P{PZ}cuc¹⁰⁶⁰⁷/CyO 12063 P{PZ}sls^rL182/TM3, Sb¹ 12285 P{PZ}Aph-4⁰⁷⁰²⁸, ry⁵⁰⁶
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External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacZ^P\T.PZ kanR ori ry^+t7.2=PZ} (FlyBase, 1996-) P{lacZ^PZ.P\T kanR ori ry^+t7.2=PZ} (FlyBase, 1996-) P{PZ} (FlyBase, 1996-, Brook et al., 1993, BDGP Project Members, 1994-1999, Treisman and Rubin, 1996, Murakami et al., 1994, Bellen et al., 2004, Castrillon et al., 1993, Parks et al., 2004, Zhang and Stankiewicz, 1998) PT-lacZ (Zhang et al., 2000) PZ (Mlodzik and Hiromi, 1992)
Secondary FlyBase IDs

References ( 41 )

Generate a list of	All references relating to this transgenic transposon ( 41 )

List References by type	Research paper ( 35 ) Supplementary material ( 1 ) Personal communication to FlyBase ( 1 ) FlyBase analysis ( 3 ) Computer file ( 1 )
Recent research papers ( 2 )
	Andersen et al., 2010, Mol. Cell. Biol. 30(19): 4744--4755 Drosophila MCRS2 Associates with RNA Polymerase II Complexes To Regulate Transcription. [FBrf0211794] Coulthard et al., 2010, Genetics 185(2): 479--495 Essential loci in centromeric heterochromatin of Drosophila melanogaster. I: the right arm of chromosome 2. [FBrf0211235] Show all research papers ...