Recombinant construct P{FRT(w^hs)}

General Information
Symbol	P{FRT(w^hs)}	FlyBase ID	FBtp0000268
Feature type	transgenic_transposon	Created / Updated	1997-03-21/1999-05-13

Size	8.341	Expression data
Location-dependent role		Associated insertions	26 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Golic and Lindquist, 1989, Ahmad and Golic, 1996)
Uses	CV term Qualifiers & info Reference Assay FLP activity mutagenesis local transposition (Golic, 1994) chromosomal aberrations (Golic, 1994) characterization w \| position effect (Ahmad and Golic, 1996) w \| PEV (Ahmad and Golic, 1996) genomic insertion screen
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	8.341
Left end	P5\' (Golic and Lindquist, 1989)
Right end	P3\' (Golic and Lindquist, 1989)

Segments	Number Orientation Symbol 1 + Car1_P5'(P5',R1) 2 + EcoRI 3 - FRT_2.1(Bc,R1) 4 + 001140 5 - w^+mW4.6(Ha3,R1) 6 + 001139 7 - FRT_0.7(Bc,H3) 8 + 001141 9 + Car1_P3'(H3,P3')
Reference	(Golic and Lindquist, 1989)
Features
	CV term Qualifiers & info Reference selectable marker (Klemenz et al., 1987, Golic, 1991, Chou and Perrimon, 1992, Golic, 1994, Kurkulos et al., 1994, Jiang and Struhl, 1995, Golic and Golic, 1996, Ahmad and Golic, 1996, Chou and Perrimon, 1996, Perrimon et al., 1996, Golic et al., 1997) chimeric gene (Klemenz et al., 1987) partial rescue (Klemenz et al., 1987) removeable cassette (Ahmad and Golic, 1996) removable cassette (Golic and Lindquist, 1989)
Component Alleles
w^+mW.hs
Reference(s)	(Klemenz et al., 1987, Jiang and Struhl, 1995, Kurkulos et al., 1994, Golic, 1994, Golic et al., 1997, Chou and Perrimon, 1992, Golic, 1991, Golic and Lindquist, 1989, Golic and Golic, 1996, Ahmad and Golic, 1996, Chou and Perrimon, 1996, Perrimon et al., 1996)
Molecular data	Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors. Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993)
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{FRT(w^hs)} (Ahmad and Golic, 1996)
descendant(s)	P{FRT(w^hs)RS3r} P{FRT(w^hs)RS5r} (Golic et al., 1997, Golic et al., 1997) P{1xFRT.G} eFRT-w.G (Ahmad and Golic, 1996, Ahmad and Golic, 1996)
Comments
	report comment: See report for Scer\FRT. (Golic and Lindquist, 1989) sequence comment: Segments FRT_2.1(Bc,R1) and FRT_0.7(Bc,H3) uncertain. (Golic and Lindquist, 1989) report comment: CAUTION: Probable errors in sequence: extent of FRT-bearing segments uncertain. (Golic and Lindquist, 1989) mapping_comment: CAUTION: Segments 3 and 7 uncertain. (Golic and Lindquist, 1989)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[>w^hs>] (Golic and Lindquist, 1989) P{>@FRT@ @w^+mW.hs@ >@FRT@=@FRT(w^hs)@} (FlyBase, 1996-) P{>FRT w^+mW.hs>FRT=>w^hs>} (FlyBase, 1996-) P{>w^hs>} (FlyBase, 1996-) P{FRT(w^hs)} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 14 )
Research paper	Golic et al., 1997, Nucleic Acids Res. 25(18): 3665--3671 FLP-mediated DNA mobilization to specific target sites in Drosophila chromosomes. [FBrf0098247] Ahmad and Golic, 1996, Genetics 144(2): 657--670 Somatic reversion of chromosomal position effects in Drosophila melanogaster. [FBrf0090428] Chou and Perrimon, 1996, Genetics 144(4): 1673--1679 The autosomal FLP-DFS technique for generating germline mosaics in Drosophila melanogaster. [FBrf0091030] Golic and Golic, 1996, Genetics 143(1): 385--400 A quantitative measure of the mitotic pairing alleles in Drosophila melanogaster and the influence of structural heterozygosity. [FBrf0087226] Perrimon et al., 1996, Genetics 144(4): 1681--1692 Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142] Jiang and Struhl, 1995, Cell 80(4): 563--572 Protein kinase A and hedgehog signaling in Drosophila limb development. [FBrf0080131] Golic, 1994, Genetics 137(2): 551--563 Local transposition of P elements in Drosophila melanogaster and recombination between duplicated elements using a site-specific recombinase. [FBrf0073185] Kurkulos et al., 1994, Genetics 136(3): 1001--1011 P element-mediated in vivo deletion analysis of white-apricot: deletions between direct repeats are strongly favored. [FBrf0073665] Heinrich et al., 1993, Dev. Genet. 14(4): 258--265 Attenuation of white gene expression in transgenic Drosophila melanogaster: possible role of a catalytic antisense RNA. [FBrf0064501] Chou and Perrimon, 1992, Genetics 131: 643--653 Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila. [FBrf0056243] Golic, 1991, Science 252: 958--961 Site-specific recombination between homologous chromosomes in Drosophila. [FBrf0055092] Golic and Lindquist, 1989, Cell 59: 499--509 The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome. [FBrf0049362] Klemenz et al., 1987, Nucleic Acids Res. 15(10): 3947--3959 The white gene as a marker in a new P-element vector for gene transfer in Drosophila. [FBrf0047108]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]

Recombinant construct P{FRT(whs)}

Recombinant construct P{FRT(w^hs)}