Recombinant construct P{enC}

General Information
Symbol	P{enC}	FlyBase ID	FBtp0000314
Feature type	transgenic_transposon

Size	13.204	Expression data	lacZ reporter
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Kassis, 1990)
Uses	CV term Qualifiers & info Reference characterization en \| regulatory (Kassis, 1990, Kassis et al., 1991) en \| transvection (Kassis et al., 1991) reporter construct en (Kassis, 1990, Kassis et al., 1991)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	13.204
Left end	P5\' (Kassis, 1990)
Right end	P3\' (Kassis, 1990)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + 001165 3 + Dvir\en_intron1 4 + 001166 5 + enA::lacZ(6.9)-F 6 + 001164 7 + Car1_P3'(H3,P3')
Reference	(Kassis, 1990)
Features
	CV term Qualifiers & info Reference chimeric gene (Kassis, 1990) partial rescue (Pirrotta, 1988) reporter gene (Kassis, 1990) selectable marker (Pirrotta, 1988, Gorman et al., 1995)
Component Alleles
Allele	Ecol\lacZ^en.C
Reference(s)	(Kassis, 1990, Kassis et al., 1992, Kassis et al., 1991)
Molecular data	Ecol\lacZ expression is governed by a fragment of en promoter extending from -2.4kb to +188bp upstream of the transcription start site. (Kassis et al., 1991, Kassis et al., 1992) Construct: Ecol\lacZ expression is governed by a fragment of en promoter extending from -2.4kb to +188bp upstream of the transcription start site. (Kassis, 1990)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Pirrotta, 1988)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information

Allele	w^en.C
Reference(s)	(Kassis et al., 1991, Kassis, 1990)
Molecular data	w expression is governed by a fragment of en promoter extending from -2.4kb to +188bp upstream of the transcription start site. Intron 1 of Dvir\en is fused to the 5' end of en and acts as a 1kb spacer between en sequences and w^+mC. (Kassis et al., 1991) Construct: w expression is governed by a fragment of en promoter extending from -2.4kb to +188bp upstream of the transcription start site. Intron 1 of Dvir\en is fused to the 5' end of en and acts as a 1kb spacer between en sequences and w^+mC. (Kassis, 1990)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage \| late neurogenic region (Kassis, 1990) embryonic stage \| mid -- embryonic stage \| late fat body primordium (Kassis, 1990) organism \| striped (Kassis, 1990)
Additional Information
Statement Reference In embryos, lower levels of expression observed in first 3 (anterior) stripes; in subsequent stripes, expression in even-numbered stripes is higher than that in odd-numbered stripes. Note: Results using assay for β-gal activity differ from those detecting the β-gal RNA. The stripe pattern persists into late stages of embryogenesis in the former case, but not in the latter. (Kassis, 1990)

Marker for
Reflects expression of	en (Kassis, 1990)
Progenitors & Descendants
progenitor(s)	pP{enC} (Kassis, 1990)
descendant(s)
Comments
	construct_comment: One of several constructs designated P{en2}, all of which contain 2.4kb of en 5\' flanking sequences and en intron 1 sequences. (Kassis et al., 1992) construct_comment: Expression observed in homozygotes versus heterozygotes; tested for proximity dependence (transvection). (Kassis et al., 1991) construct_comment: One of a series of constructs analyzed for effects of en sequences upon expression of the adjacent w^+mC gene. (Kassis et al., 1991) construct_comment: One of a series of constructs used to characterize en regulatory elements contained within the first intron. (Kassis, 1990)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{en2} (FlyBase, 1996-) P{enC} (FlyBase, 1996-) P{w^+mC lacZ^en.C=enC} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 3 )
Research paper	Kassis et al., 1992, Proc. Natl. Acad. Sci. U.S.A. 89: 1919--1923 Altering the insertional specificity of a Drosophila transposable element. [FBrf0057521] Kassis et al., 1991, Genetics 128: 751--761 A fragment of engrailed regulatory DNA can mediate transvection of the white gene in Drosophila. [FBrf0054180] Kassis, 1990, Genes Dev. 4: 433--443 Spatial and temporal control elements of the Drosophila engrailed gene. [FBrf0051851]