Recombinant construct P{NM}

General Information
Symbol	P{NM}	FlyBase ID	FBtp0000350
Feature type	transgenic_transposon	Created / Updated	1997-03-21/2001-12-17

Size	7.9	Expression data
Location-dependent role		Associated insertions	5 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Xu and Rubin, 1993)
Uses	CV term Qualifiers & info Reference subcellular target membrane \| heat-shock-induced (Xu and Rubin, 1993) heat-shock construct N (Xu and Rubin, 1993) analytical tool mosaic analysis (Xu and Rubin, 1993)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	7.9 (Xu and Rubin, 1993)
Left end	P5\' (Xu and Rubin, 1993)
Right end	P3\' (Xu and Rubin, 1993)

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference cell marker (Xu and Rubin, 1993) epitope tag (Xu and Rubin, 1993) chimeric gene (Xu and Rubin, 1993) selectable marker (Xu and Rubin, 1993, Schweisguth, 1995, Boutros et al., 1998) subcellularly targeted (Xu and Rubin, 1993) molecular tag (Xu and Rubin, 1993) inducible promoter (Xu and Rubin, 1993)
Component Alleles
N^{NM.hs.T:Hsap\MYC}
Reference(s)	(Xu and Rubin, 1993, Xu et al., 1995, Schweisguth, 1995, Johnston et al., 1998)
Molecular data	Construct: Myc peptide tagged to amino acids 1-85 and 1466-1963 of the N coding sequence, to allow marking of the cell membrane, expressed from an Hsp70 promoter. (Xu and Rubin, 1993)
Phenotypic class
Phenotype manifest in
Other information
w^+mC
Reference(s)	(Xu and Rubin, 1993, Schweisguth, 1995, Boutros et al., 1998)
Molecular data	Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors. 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Minigene of the white cDNA. (Chung et al., 1993) Deletion of w^+mC 5' flanking sequences to position -305. (Qian and Pirrotta, 1995)
Phenotypic class	courtship defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{NM} (Xu and Rubin, 1993)
descendant(s)
Comments
	construct_comment: N^NM.hs.T:Myc protein is primarily associated with plasma and ER membranes; visualized using monoclonal antibody against T:Hsap\MYC epitope. (Xu and Rubin, 1993)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[mini-w⁺; hs-NM] P[mini-w⁺, hs-NM] (Xu and Rubin, 1993) P{@w^+mC@ @N^NM.hs.T:Myc@=@NM@} (FlyBase, 1996-) P{NM} (FlyBase, 1996-) P{w^+mC N^NM.hs.T:Myc=NM} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 17 )
Research paper	Giordano et al., 2002, Genetics 160(2): 637--648 RNAi triggered by symmetrically transcribed transgenes in Drosophila melanogaster. [FBrf0144904] Lloyd et al., 2002, Genome 45(2): 296--312 Enhancer of garnet/δAP-3 is a cryptic allele of the white gene and identifies the intracellular transport system for the white protein. [FBrf0147069] Soldatov et al., 1999, Molec. Cell. Biol. 19(5): 3769--3778 TAFII40 protein is encoded by the e(y)1 gene: biological consequences of mutations. [FBrf0108279] Boutros et al., 1998, Cell 94(1): 109--118 Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling. [FBrf0103240] Johnston et al., 1998, Genetics 149(1): 131--142 The homeobox gene cut interacts genetically with the homeotic genes proboscipedia and Antennapedia. [FBrf0102837] Hagstrom et al., 1996, Genes Dev. 10(24): 3202--3215 Fab-7 functions as a chromatin domain boundary to ensure proper segment specification by the Drosophila bithorax complex. [FBrf0091065] Hing and Carlson, 1996, J. Neurobiol. 30(4): 454--464 Male-male courtship behavior induced by ectopic expression of the Drosophila white gene: Role of sensory function and age. [FBrf0089680] True et al., 1996, Genetics 142(3): 819--837 A genome-wide survey of hybrid incompatibility factors by the introgression of marked segments of Drosophila mauritiana chromosomes into Drosophila simulans. [FBrf0086664] Qian and Pirrotta, 1995, Genetics 139(2): 733--744 Dosage compensation of the Drosophila white gene requires both the X chromosome environment and multiple intragenic elements. [FBrf0080335] Schweisguth, 1995, Development 121(6): 1875--1884 Suppressor of Hairless is required for signal reception during lateral inhibition in the Drosophila pupal notum. [FBrf0079375] Xu et al., 1995, Development 121(4): 1053--1063 Identifying tumor suppressors in genetic mosaics: the Drosophila lats gene encodes a putative protein kinase. [FBrf0079656] Dorer and Henikoff, 1994, Cell 77(7): 993--1002 Expansions of transgene repeats cause heterochromatin formation and gene silencing in Drosophila. [FBrf0072979] Chung et al., 1993, Cell 74(3): 505--514 A 5' element of the chicken -globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila. [FBrf0064408] Roseman et al., 1993, EMBO J. 12(2): 435--442 The su(Hw) protein insulates expression of the Drosophila melanogaster white gene from chromosomal position-effects. [FBrf0058161] Xu and Rubin, 1993, Development 117(4): 1223--1237 Analysis of genetic mosaics in developing and adult Drosophila tissues. [FBrf0058070]
Review	Pirrotta, 1988, Rodriguez, Denhardt, 1988: 437--456 Vectors for P-mediated transformation in Drosophila. [FBrf0048982]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]