Recombinant construct P{GawB}

General Information
Symbol	P{GawB}	FlyBase ID	FBtp0000352
Feature type	transgenic_transposon

Size	11.279	Expression data	GAL4 reporter/driver
Associated insertions	5371 available
Molecular map
Recent Updates
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FB2013_03	Alleles Scer\GAL4[071] crc[E8] Kdm2[C774] Scer\GAL4[333-tio] Scer\GAL4[C829] Scer\GAL4[11Y] CG33158[169Y] CG12316[11Y] Scer\GAL4[394] Scer\GAL4[369] Scer\GAL4[483] corto[369] Scer\GAL4[169Y] egh[C502a] aos[169Y] aay[113] Scer\GAL4[NP0368] Scer\GAL4[Dll-MD713] Scer\GAL4[385] Scer\GAL4[crc-E8] Scer\GAL4[C502a] ewg[001] Moe[003] Scer\GAL4[003] Scer\GAL4[470] Scer\GAL4[383] Scer\GAL4[C60] Hsp23[C404] Scer\GAL4[bl-NP3591] CG7377[383] Scer\GAL4[Act.NP3121] Scer\GAL4[001] Scer\GAL4[402] Scer\GAL4[C318a] Scer\GAL4[134] wds[C502a] Scer\GAL4[010] Scer\GAL4[499] Scer\GAL4[123] Hr39[C318a] RasGAP1[c255] CG11629[496] Dll[MD713] crc[G74] Scer\GAL4[C560] Scer\GAL4[113] Scer\GAL4[C552] Scer\GAL4[C115] Scer\GAL4[496] Scer\GAL4[C689] Scer\GAL4[102Y] tio[333] Alh[010] her[C115] Scer\GAL4[crc-G74] stich1[463] grp[C115] Scer\GAL4[C404] MESR3[470] Scer\GAL4[463] Scer\GAL4[C800] Scer\GAL4[103] Scer\GAL4[C774] arg[203Y] lola[123] rt[383] Insertions P{GawB}Alh[010] P{GawB}rt[383] P{GawB}crc[E8] P{GawB}102Y P{GawB}071 P{GawB}394 P{GawB}crc[G74] P{GawB}Hsp23[C404] P{GawB}aos[169Y] P{GawB}483 P{GawB}385 P{GawB}C552 P{GawB}Kdm2[C774] P{GawB}aay[113] P{GawB}ewg[001] P{GawB}Dll[MD713] P{GawB}C829 P{GawB}Moe[003] P{GawB}C60 P{GawB}CG12316[11Y] P{GawB}tio[333] P{GawB}CG11629[496] P{GawB}corto[369] P{GawB}grp[C115] P{GawB}Hr39[C318a] P{GawB}C560 P{GawB}C800 P{GawB}499 P{GawB}134 P{GawB}103 P{GawB}C689 P{GawB}MESR3[470] P{GawB}wds[C502a] P{GawB}lola[123] P{GawB}402 P{GawB}stich1[463]
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role	GAL4 driver/enhancer trap (Brand and Perrimon, 1993)
Description	CV term Qualifiers & info Reference transposon P-element (Brand and Perrimon, 1993, Hayashi et al., 2002)
Uses	CV term Qualifiers & info Reference carrier transposon binary system \| GAL4 (Brand and Perrimon, 1993) P-element (Brand and Perrimon, 1993) enhancer trap binary system (Brand and Perrimon, 1993) P-element (Brand and Perrimon, 1993) genomic insertion screen
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	11.279
Left end	P5\' (Brand and Perrimon, 1993)
Right end	P3\' (Brand and Perrimon, 1993)

Segments	Number Orientation Symbol 1 + GawB_P5'(P5',Bg2) 2 + 001152 3 + 001005 4 + w^+mW4.6(Ha3,R1) 5 + 001006 6 - pBL2M-mcs(K,Sc1) 7 + 001153 8 + Car1_P3'(H3,P3')
Reference	(Brand and Perrimon, 1993)
Features
	CV term Qualifiers & info Reference bacterial selection (Wilson et al., 1989) binary component (Brand and Perrimon, 1993, Brand et al., 1994) chimeric gene (Klemenz et al., 1987) chimeric gene (Brand and Perrimon, 1993) chimeric gene (Brand et al., 1994) enhancer trap (Brand and Perrimon, 1993, Brand et al., 1994) partial rescue (Klemenz et al., 1987) reporter gene (Brand and Perrimon, 1993) selectable marker (Klemenz et al., 1987)
Component Alleles
Allele	Scer\GAL4^wB
Reference(s)	(Brand and Perrimon, 1993, Mollereau et al., 2000, Hatton et al., 1998, Yoshihara and Ito, 2000, Suh et al., 2008)
Molecular data	Construct: The Ecol\lacZ enhancer construct pP{lwB} has been modified to encode Scer\GAL4 instead of Ecol\lacZ. (Brand and Perrimon, 1993)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mW.hs
Reference(s)	(Klemenz et al., 1987, Brand and Perrimon, 1993)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference third instar larval stage embryonic/larval salivary gland (Gerlitz et al., 2002)
Additional Information
Statement Reference Pattern of expression dependent upon position of insertion. (Brand and Perrimon, 1993)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{GawB} (Brand and Perrimon, 1993)
descendant(s)	P{3'GawB} (Marcellini et al., 2005) P{5'GawB} (FlyBase, 1992-) P{?'GawB} (Houot et al., 2010) P{?GawB} (Grosjean et al., 2001) P{GAL4E69} (Adachi-Yamada, 2002) P{GawB.w-} (An et al., 2000) P{GawB.ΔS} (Thor, 2003.11) P{GawB-wex1} (Murray et al., 2006) P{GawB-Δ2.G} (Grosjean et al., 2001) P{GawB-Δ3.G} (Grosjean et al., 2001) P{GawB-Δ8.G} (Grosjean et al., 2001) P{GawB-Δ11.G} (Grosjean et al., 2001) P{GawB-Δ13.G} (Grosjean et al., 2001) P{GawB-Δ24.G} (Grosjean et al., 2001) P{GawB-Δ27.G} (Grosjean et al., 2001) P{GawB-Δ61.G} (Grosjean et al., 2001) P{GawB-Δ69.G} (Grosjean et al., 2001) P{GawB-Δ78.G} (Grosjean et al., 2001) P{GawB-Δ79.G} (Grosjean et al., 2001) P{GawB-ΔKE} (Edwards, 2004.12) P{LGawB} (Lai et al., 2008) P{Switch1} (Roman et al., 2001)
Comments
	report comment: CAUTION: Probable minor errors in compiled sequence, especially at junctions. (Brand and Perrimon, 1993) mapping_comment: CAUTION: Junctions 001152 and 001154 not certain. (Brand and Perrimon, 1993) sequence comment: CAUTION: Probable minor errors in compiled sequence, especially at junctions 001152 and 001154. (Brand and Perrimon, 1993)
Stocks Listed in FlyBase ( 4070 )
Bloomington	5076 P{GawB}T155 33807 P{GawB}C164 6927 w^; P{GawB}4G 7148 w^; P{GawB}43 1774 w^; P{GawB}69B 1782 w^; P{GawB}32B 1874 w^; P{GawB}389 2683 w^; P{GawB}V55 2703 P{GawB}C23, w^* 4935 w^; P{GawB}48Y 7149 w^; P{GawB}227
Kyoto	108022 P{GawB}T155
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External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[GAL4;w⁺] (Ferveur et al., 1995) P{GAL4} (FlyBase, 1996-) P{GAL4^wB w^+mW.hs ampR ori=GawB} (FlyBase, 1996-) P{GawB} (FlyBase, 1996-, Hayashi et al., 2002, Yang et al., 1995, Sozen et al., 1997, Siegmund and Korge, 2001, Manseau et al., 1997, van Roessel and Brand, 2000) pGawB (Suh et al., 2008) pGawB insertionEnhancer Trap GAL4 (Brand and Perrimon, 1993)
Secondary FlyBase IDs

References ( 127 )

Generate a list of	All references relating to this transgenic transposon ( 127 )

List References by type	Research paper ( 105 ) Supplementary material ( 2 ) Review ( 1 ) Personal communication to FlyBase ( 15 ) Abstract ( 1 ) FlyBase analysis ( 3 )
Recent research papers ( 14 )
	Eddison et al., 2012, PLoS ONE 7(12): e51684 A genetic screen for olfactory habituation mutations in Drosophila: analysis of novel foraging alleles and an underlying neural circuit. [FBrf0220425] Timofeev et al., 2012, Neuron 75(1): 80--93 Localized netrins act as positional cues to control layer-specific targeting of photoreceptor axons in Drosophila. [FBrf0218874] Eddison et al., 2011, Neuron 70(5): 979--990 arouser Reveals a Role for Synapse Number in the Regulation of Ethanol Sensitivity. [FBrf0213908] Gallio et al., 2011, Cell 144(4): 614--624 The coding of temperature in the Drosophila brain. [FBrf0213085] Kaun et al., 2011, Nat. Neurosci. 14(5): 612--619 A Drosophila model for alcohol reward. [FBrf0213574] Lamaze et al., 2011, EMBO Rep. 12(6): 549--557 The E3 ubiquitin ligase CTRIP controls CLOCK levels and PERIOD oscillations in Drosophila. [FBrf0213851] Nakagawa et al., 2011, Mech. Dev. 128(5-6): 258--267 Spatial and temporal requirement of Defective proventriculus activity during Drosophila midgut development. [FBrf0214038] Ng et al., 2011, Curr. Biol. 21(8): 625--634 Glial cells physiologically modulate clock neurons and circadian behavior in a calcium-dependent manner. [FBrf0213518] Pappu et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(18): 7571--7576 Robo-3-mediated repulsive interactions guide R8 axons during Drosophila visual system development. [FBrf0213605] Pueyo and Couso, 2011, Dev. Biol. 355(2): 183--193 Tarsal-less peptides control Notch signalling through the Shavenbaby transcription factor. [FBrf0213970] Tian et al., 2011, Nat. Neurosci. 14(10): 1267--1275 Drosophila Rae1 controls the abundance of the ubiquitin ligase Highwire in post-mitotic neurons. [FBrf0216253] Tomita et al., 2011, J. Neurosci. 31(37): 13137--13146 Pan-Neuronal Knockdown of Calcineurin Reduces Sleep in the Fruit Fly, Drosophila melanogaster. [FBrf0215571] Tripura et al., 2011, Int. J. Dev. Biol. 55(6): 583--590 Regulation and activity of JNK signaling in the wing disc peripodial membrane during adult morphogenesis in Drosophila. [FBrf0216296] Vrailas-Mortimer et al., 2011, Dev. Cell 21(4): 783--795 A Muscle-Specific p38 MAPK/Mef2/MnSOD Pathway Regulates Stress, Motor Function, and Life Span in Drosophila. [FBrf0216446] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...