Recombinant construct P{GawB}

General Information
Symbol	P{GawB}	FlyBase ID	FBtp0000352
Feature type	transgenic_transposon

Size	11.279	Expression data	GAL4 reporter/driver
Associated insertions	5286 available
Molecular map
Recent Updates
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FB2011_10	Stocks y[1] w[1118]; P{w[+m]=GawB}GH146 w[] P{w[+mW.hs]=GawB}GH86 Insertions P{GawB}GH298 P{GawB}GH327 P{GawB}GH146 P{GawB}GH336 P{GawB}GH320 P{GawB}E409 References Kadowaki, 2011.10.9, P{GawB}E409. Alleles Eip63F-1[55B] Scer\GAL4[GH336] Scer\GAL4[puc-E69-H] Scer\GAL4[puc-E69-A] Scer\GAL4[NP0863] Scer\GAL4[GH146] Scer\GAL4[GH320] Scer\GAL4[GH327] Neu3[c768] Scer\GAL4[puc-E69-K] CG11943[179Y] Scer\GAL4[puc-E69-G] Scer\GAL4[puc-E69-M] Scer\GAL4[puc-E69-Q] Scer\GAL4[GH298] Scer\GAL4[puc-E69-F] Scer\GAL4[puc-E69-S] Scer\GAL4[E409] Scer\GAL4[puc-E69-E] Scer\GAL4[puc-E69-I] Scer\GAL4[puc-E69-C]
FB2012_01	Insertions P{GawB}1501 P{GawB}p38b[83] P{GawB}MT11 P{GawB}MT14 P{GawB}MD758 P{GawB}N[MD776] P{GawB}puc[E69-unk] Alleles Scer\GAL4[puc-E69-unk] eIF-2gamma[NP3153] Scer\GAL4[NP0282] Scer\GAL4[MT14] N[MD776] Scer\GAL4[1501] Scer\GAL4[CG11873-NP2646] Scer\GAL4[Ent2-NP7068] Scer\GAL4[p38b-83] Scer\GAL4[MT11] Scer\GAL4[nab-NP3537] CG11873[NP2646] Scer\GAL4[wg-MD758] Scer\GAL4[daw-NP4661] Scer\GAL4[Ilp6-NP1079] Scer\GAL4[NP3153] References Vrailas-Mortimer et al., 2011, Dev. Cell 21(4): 783--795 Lamaze et al., 2011, EMBO Rep. 12(6): 549--557 Gendre and Stocker, 2011.11.27, Stocker insertions. FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Hatton-Ellis and Simpson, 2011.10.26, P{GawB}N[MD776]. Tripura et al., 2011, Int. J. Dev. Biol. 55(6): 583--590 Stocks y[1] w[]; P{w[+mW.hs]=GawB}C-765 y[1] w[]; P{w[+mW.hs]=FRT(w[hs])}G13 P{w[+mW.hs]=GawB}GH146 P{w[+mC]=UAS-mCD8::GFP.L}LL5 Chi[e5.5]/CyO w[]; P{w[+mW.hs]=GawB}E409 y[1] w[] P{w[+mW.hs]=GawB}fz3[MD810]; P{w[+mC]=UAS-y.C}MC1 w[]; P{w[+mW.hs]=GawB}sr[md710]/TM6B, Tb[1] y[1] w[], P{w[+mW.hs]=GawB}N[MD776]/FM6 y[1] P{w[+mW.hs]=GawB}CG32816[NP6014] P{GawB}ac[sbm] P{w[+mW.hs]=GawB}MD758/CyO; P{w[+mC]=UAS-y.C}MC1 w[]; P{w[+mW.hs]=GawB}MD806/TM6B, Tb[1] Lim1[E9] P{ry[+t7.2]=neoFRT}19A/FM7c; P{w[+mW.hs]=GawB}GH146 P{w[+mC]=UAS-mCD8::GFP.L}LL5/CyO ap[4]/CyO, P{w[+mC]=ActGFP}JMR1; P{w[+mC]=GawB}EM461 w[]; P{w[+mW.hs]=GawB}ush[MD751], P{w[+mC]=UAS-GFP.S65T}Myo31DF[T2] w[*]; P{w[+mW.hs]=GawB}GH298 y[1] P{w[+mW.hs]=GawB}CG32816[NP6014] ac[cami]
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	GAL4 driver/enhancer trap (Brand and Perrimon, 1993)
Description	CV term Qualifiers & info Reference transposon P-element (Brand and Perrimon, 1993, Hayashi et al., 2002)
Uses	CV term Qualifiers & info Reference carrier transposon binary system \| GAL4 (Brand and Perrimon, 1993) P-element (Brand and Perrimon, 1993) enhancer trap binary system (Brand and Perrimon, 1993) P-element (Brand and Perrimon, 1993) genomic insertion screen
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	11.279
Left end	P5\' (Brand and Perrimon, 1993)
Right end	P3\' (Brand and Perrimon, 1993)

Segments	Number Orientation Symbol 1 + GawB_P5'(P5',Bg2) 2 + 001152 3 + 001005 4 + w^+mW4.6(Ha3,R1) 5 + 001006 6 - pBL2M-mcs(K,Sc1) 7 + 001153 8 + Car1_P3'(H3,P3')
Reference	(Brand and Perrimon, 1993)
Features
	CV term Qualifiers & info Reference bacterial selection (Wilson et al., 1989) binary component (Brand and Perrimon, 1993, Brand et al., 1994) chimeric gene (Klemenz et al., 1987) chimeric gene (Brand and Perrimon, 1993) chimeric gene (Brand et al., 1994) enhancer trap (Brand and Perrimon, 1993, Brand et al., 1994) partial rescue (Klemenz et al., 1987) reporter gene (Brand and Perrimon, 1993) selectable marker (Klemenz et al., 1987)
Component Alleles
Allele	Scer\GAL4^wB
Reference(s)	(Brand and Perrimon, 1993, Mollereau et al., 2000, Hatton et al., 1998, Yoshihara and Ito, 2000, Suh et al., 2008)
Molecular data	Construct: The Ecol\lacZ enhancer construct pP{lwB} has been modified to encode Scer\GAL4 instead of Ecol\lacZ. (Brand and Perrimon, 1993)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mW.hs
Reference(s)	(Klemenz et al., 1987, Brand and Perrimon, 1993)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference third instar larval stage embryonic/larval salivary gland (Gerlitz et al., 2002)
Additional Information
Statement Reference Pattern of expression dependent upon position of insertion. (Brand and Perrimon, 1993)

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{GawB} (Brand and Perrimon, 1993)
descendant(s)	P{3'GawB} (Marcellini et al., 2005) P{5'GawB} (FlyBase, 1992-) P{?'GawB} (Houot et al., 2010) P{?GawB} (Grosjean et al., 2001) P{GAL4E69} (Adachi-Yamada, 2002) P{GawB.w-} (An et al., 2000) P{GawB.ΔS} (Thor, 2003.11) P{GawB-wex1} (Murray et al., 2006) P{GawB-Δ2.G} (Grosjean et al., 2001) P{GawB-Δ3.G} (Grosjean et al., 2001) P{GawB-Δ8.G} (Grosjean et al., 2001) P{GawB-Δ11.G} (Grosjean et al., 2001) P{GawB-Δ13.G} (Grosjean et al., 2001) P{GawB-Δ24.G} (Grosjean et al., 2001) P{GawB-Δ27.G} (Grosjean et al., 2001) P{GawB-Δ61.G} (Grosjean et al., 2001) P{GawB-Δ69.G} (Grosjean et al., 2001) P{GawB-Δ78.G} (Grosjean et al., 2001) P{GawB-Δ79.G} (Grosjean et al., 2001) P{GawB-ΔKE} (Edwards, 2004.12) P{LGawB} (Lai et al., 2008) P{Switch1} (Roman et al., 2001)
Comments
	report comment: CAUTION: Probable minor errors in compiled sequence, especially at junctions. (Brand and Perrimon, 1993) mapping_comment: CAUTION: Junctions 001152 and 001154 not certain. (Brand and Perrimon, 1993) sequence comment: CAUTION: Probable minor errors in compiled sequence, especially at junctions 001152 and 001154. (Brand and Perrimon, 1993)
Stocks Listed in FlyBase ( 4066 )
Bloomington	5076 P{GawB}T155 33807 P{GawB}C164 6927 w^; P{GawB}4G 7148 w^; P{GawB}43 1774 w^; P{GawB}69B 1782 w^; P{GawB}32B 1874 w^; P{GawB}389 2683 w^; P{GawB}V55 2703 P{GawB}C23, w^* 4935 w^; P{GawB}48Y 7149 w^; P{GawB}227
Kyoto	108022 P{GawB}T155
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External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[GAL4;w⁺] (Ferveur et al., 1995) P{GAL4} (FlyBase, 1996-) P{GAL4^wB w^+mW.hs ampR ori=GawB} (FlyBase, 1996-) P{GawB} (FlyBase, 1996-, Hayashi et al., 2002, Yang et al., 1995, Sozen et al., 1997, Siegmund and Korge, 2001, Manseau et al., 1997, van Roessel and Brand, 2000) pGawB (Suh et al., 2008) pGawB insertionEnhancer Trap GAL4 (Brand and Perrimon, 1993)
Secondary FlyBase IDs

References ( 120 )

Generate a list of	All references relating to this transgenic transposon ( 120 )

List References by type	Research paper ( 99 ) Review ( 1 ) Personal communication to FlyBase ( 15 ) Abstract ( 1 ) FlyBase analysis ( 4 )
Recent research papers ( 21 )
	Eddison et al., 2011, Neuron 70(5): 979--990 arouser Reveals a Role for Synapse Number in the Regulation of Ethanol Sensitivity. [FBrf0213908] Gallio et al., 2011, Cell 144(4): 614--624 The coding of temperature in the Drosophila brain. [FBrf0213085] Lamaze et al., 2011, EMBO Rep. 12(6): 549--557 The E3 ubiquitin ligase CTRIP controls CLOCK levels and PERIOD oscillations in Drosophila. [FBrf0213851] Nakagawa et al., 2011, Mech. Dev. 128(5-6): 258--267 Spatial and temporal requirement of Defective proventriculus activity during Drosophila midgut development. [FBrf0214038] Ng et al., 2011, Curr. Biol. 21(8): 625--634 Glial cells physiologically modulate clock neurons and circadian behavior in a calcium-dependent manner. [FBrf0213518] Pappu et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(18): 7571--7576 Robo-3-mediated repulsive interactions guide R8 axons during Drosophila visual system development. [FBrf0213605] Tripura et al., 2011, Int. J. Dev. Biol. 55(6): 583--590 Regulation and activity of JNK signaling in the wing disc peripodial membrane during adult morphogenesis in Drosophila. [FBrf0216296] Vrailas-Mortimer et al., 2011, Dev. Cell 21(4): 783--795 A Muscle-Specific p38 MAPK/Mef2/MnSOD Pathway Regulates Stress, Motor Function, and Life Span in Drosophila. [FBrf0216446] Céspedes et al., 2010, PLoS ONE 5(11): e15382 Dioxin Toxicity In Vivo Results from an Increase in the Dioxin-Independent Transcriptional Activity of the Aryl Hydrocarbon Receptor. [FBrf0212269] de Navascués and Modolell, 2010, Mech. Dev. 127(9-12): 393--406 The pronotum LIM-HD gene tailup is both a positive and a negative regulator of the proneural genes achaete and scute of Drosophila. [FBrf0212072] Houot et al., 2010, Genetics 185(4): 1297--1309 The Consequences of Regulation of desat1 Expression for Pheromone Emission and Detection in Drosophila melanogaster. [FBrf0211531] Masuda-Nakagawa et al., 2010, Gene Expr. Patterns 10(7-8): 328--337 Targeting expression to projection neurons that innervate specific mushroom body calyx and antennal lobe glomeruli in larval Drosophila. [FBrf0211879] Morrison and Halder, 2010, genesis 48(1): 3--7 Characterization of a Dorsal-Eye Gal4 Line in Drosophila. [FBrf0209713] Okumura et al., 2010, Dev. Biol. 344(2): 693--706 Left-right asymmetric morphogenesis of the anterior midgut depends on the activation of a non-muscle myosin II in Drosophila. [FBrf0211383] Pines et al., 2010, Development 137(6): 913--922 The cytolinker Pigs is a direct target and a negative regulator of Notch signalling. [FBrf0210124] Potter and Luo, 2010, PLoS ONE 5(4): e10168 Splinkerette PCR for mapping transposable elements in Drosophila. [FBrf0210616] Siekhaus et al., 2010, Nat. Cell Biol. 12(6): 605--610 RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila. [FBrf0210888] Venkatesh and Shyamala, 2010, J. Genet. 89(4): e38--e42 GAL4 enhancer trap strains with reporter gene expression during the development of adult brain in Drosophila melanogaster. [FBrf0212889] Young and Armstrong, 2010, J. Comp. Neurol. 518(9): 1500--1524 Structure of the adult central complex in Drosophila: Organization of distinct neuronal subsets. [FBrf0210154] Yu et al., 2010, Curr. Biol. 20(18): 1602--1614 Cellular Organization of the Neural Circuit that Drives Drosophila Courtship Behavior. [FBrf0211884] Zeng et al., 2010, genesis 48(10): 607--611 Characterization of midgut stem cell- and enteroblast-specific Gal4 lines in drosophila. [FBrf0212138] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...