Recombinant construct P{UAS-lacZ.B}

General Information
Symbol	P{UAS-lacZ.B}	FlyBase ID	FBtp0000355
Feature type	transgenic_transposon

Size	9.756	Expression data	lacZ reporter
Associated insertions	10 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Brand and Perrimon, 1993)
Uses	CV term Qualifiers & info Reference binary system (targeted) Ecol\lacZ (Brand and Perrimon, 1993) reporter construct Ecol\lacZ \| binary system (Brand and Perrimon, 1993)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	9.756
Left end
Right end

Segments	Number Orientation Symbol 1 + Car1_P5'(P5',R1) 2 - 001069 3 - w^+mC4.1(Sp) 4 - 001068 5 + 001187 6 - Hsp70Bb::lacZ::SV40_4.6 7 - 5xUAS 8 + 001186 9 + Car1_P3'(H3,P3')
Reference	(Brand et al., 1994)
Features
	CV term Qualifiers & info Reference binary component (Brand and Perrimon, 1993) chimeric gene (Brand and Perrimon, 1993) partial rescue (Pirrotta, 1988) reporter gene (Brand and Perrimon, 1993) selectable marker (Pirrotta, 1988, Brand and Perrimon, 1993, Muller, 1995)
Component Alleles
Allele	Ecol\lacZ^Scer\UAS.cBa
Reference(s)	(Brand and Perrimon, 1993, Apitz et al., 2005, Liu et al., 2007, Kolesnikov and Beckendorf, 2005, Poirier et al., 2008, Kidd and Lieber, 2002, Kusch and Reuter, 1999, Han et al., 2000, Gorfinkiel et al., 1999, McBride et al., 1999, Tracey et al., 2000, Yang et al., 2000, Sapir et al., 1998, Robertson et al., 2003, Shigenaga et al., 1997, Klapper et al., 2002, Renaud and Simpson, 2001, Van Doren et al., 1998, Heberlein et al., 1998, Hewes et al., 2003, Hu and Castelli-Gair, 1999, Heimbeck et al., 2001, Roman et al., 2001, Sepp et al., 2001, Renn et al., 1999, Harden et al., 2002, Iwaki and Lengyel, 2002, Takaesu et al., 2002, Alfonso and Jones, 2002, Nakagoshi et al., 2002, Sharma et al., 2002, Roman and Davis, 2002, Savarit and Ferveur, 2002, Manoli and Baker, 2004, Armstrong et al., 2002, Brand et al., 1994, Englund et al., 2002, Cadigan et al., 2002, Gendre et al., 2004, Frasch, 1995, Park et al., 2004, Staehling-Hampton et al., 1994, Johnson et al., 2003, Gomez-Diaz et al., 2004, Chang et al., 2000, Wang et al., 2003, Park et al., 2000, Fuse et al., 1994, Monastirioti, 2003, Cooper et al., 2000, Deuring et al., 2000, Allen et al., 1993, Kramer et al., 1995, Featherstone et al., 2000, Zhao et al., 2000, Lei et al., 2003, Brand and Perrimon, 1994, Kraus and Lis, 1994, Scholz et al., 2000, Kolonin and Finley, 2000, Sandrelli et al., 2001, Feany and Bender, 2000, Kaneko and Hall, 2000, Sung and Robinow, 2000, Mantrova et al., 1999, Balakireva et al., 2000, Shyamala and Chopra, 1999, Chao and Nagoshi, 1999, Torroja et al., 1999, Sanson et al., 1999, Varadarajan and VijayRaghavan, 1999, Labourier et al., 1999, Namba and Minden, 1999, Kopp et al., 1999, Lee and Luo, 1999, Heimbeck et al., 1999, Wing et al., 1998, Brodu et al., 1999, Rorth, 1998, Moreau-Fauvarque et al., 1998, Jennings et al., 1999, de Celis et al., 1999, Rincon-Limas et al., 1999, Orihara et al., 1999, Ruden et al., 1999, Brand, 1997.6.30, Klapper, 2000, Deng and Bownes, 1997, Tettamanti et al., 1997, Roy and VijayRaghavan, 1997, Rosay et al., 1997, Sanchez et al., 1997, Casares et al., 1997, Furriols and Bray, 2000, Shigenaga et al., 1997, McNabb et al., 1997, Reddy et al., 1997, Ito et al., 1997, Curtiss and Heilig, 1997, Plautz et al., 1997, Tsai et al., 2004, Apitz et al., 2004, Dubois et al., 2001, Venkatesh et al., 2001, Marquez et al., 2001, Miller et al., 2001, Wing et al., 2001, Boivin and Dura, 1998, Campbell and Tomlinson, 1998, Akiyama-Oda et al., 1998, Cadigan et al., 1998, Klapper et al., 2001, Melzig et al., 1998, Kraft et al., 1998, Sun and Wyman, 1996, Rousset et al., 2001, Lee et al., 1996, Heitzler et al., 1996, Hidalgo et al., 1995, Stortkuhl and Kettler, 2001, Allen et al., 1998, Shiga et al., 1996, Flores-Saaib et al., 2001, Roy and VijayRaghavan, 1998, Lecuit et al., 1996, Yoshihara and Ito, 2000, Baylies et al., 1995, Guillemin et al., 1996, Bernardoni et al., 1998, Zhang and Carthew, 1998, O'Dell et al., 1995, Yoffe et al., 1995, Brand and Dormand, 1995, Glossop and Shepherd, 1998, Balakireva et al., 1998, Borod and Heberlein, 1998, Schupbach and Wieschaus, 1998, McCall and Bender, 1996, Gustafson and Boulianne, 1996, Kim et al., 1995, Baker and Schubiger, 1996, Zink and Paro, 1995, Ito et al., 1995, Smith and Shepherd, 1996, Phelan et al., 1996, Yeh et al., 1995, Yang et al., 1995, Johnson et al., 1995, Baker et al., 1996, Couso et al., 1995, Timmons et al., 1997, Scholz et al., 1997, Smith et al., 1996, Jimenez and Ish-Horowicz, 1997, Halfon et al., 1997, Budnik et al., 1996, Cambridge et al., 1997, Zhou et al., 1997, Holz et al., 2003, Roy et al., 1997, Seugnet et al., 1997, Anant et al., 1998, Sozen et al., 1997, Milan et al., 1997, Nakayama et al., 1997, Phillis et al., 1996, Bellaiche et al., 1996, Connolly et al., 1996, Crew et al., 1997, Larkin et al., 1996, Scholz et al., 1997, Stocker et al., 1997, Jonsson and Knust, 1996, Leevers et al., 1996, Huang and Fischer-Vize, 1996, Tissot et al., 1997, Pignoni and Zipursky, 1997, Morimura et al., 1996, Cambridge and Minden, 1996, Freeman, 1996, Chanut and Heberlein, 1997, Casares et al., 1996, Xiao et al., 1996, Freeman, 1997.3.15, Kaneko, 1997.3.14, Van Doren, 1997.3.14, Yang, 1997.3.14, Calleja et al., 1996, Morimoto et al., 1996, Elfring et al., 1998, Sturtevant et al., 1997, Sutherland et al., 1996, Audibert et al., 1998, Georgias et al., 1997, Joiner and Griffith, 1997, Suzanne et al., 2003, Fang et al., 2006, Wolfstetter et al., 2009, Kim et al., 2006, Vanderzwan-Butler et al., 2007, Pereanu et al., 2007, Guan et al., 2007, Bejarano et al., 2005, Chan et al., 2007, Kennerdell and Carthew, 2000, Ford et al., 2007, Duan et al., 2007, Kazemi-Esfarjani and Benzer, 2002, Wang and Huang, 2009, Barrett et al., 2008, An et al., 2000, Benna et al., 2010, Paladi and Tepass, 2004, Tokusumi et al., 2007, Armstrong et al., 1998, Rhiner et al., 2010, Marinho et al., 2011, Sellami et al., 2010, Velichkova et al., 2010, Otsuna and Ito, 2006, Jacobsson et al., 2009, Nguyen et al., 2007, Yang et al., 2011, Deng et al., 2010, Lahr et al., 2012, Jean et al., 2012, de Haro et al., 2006, Chang et al., 2008, Mershin et al., 2004, Wang et al., 2011)
Molecular data	Ecol\lacZ is expressed under the control of Scer\UAS regulatory sequences. (Ito et al., 1995, Gustafson and Boulianne, 1996) Ecol\lacZ expression driven from an Hsp70 minimal promoter and governed by tandemly arrayed, optimized UAS Scer\GAL4 binding sites. (Brand and Perrimon, 1993) Construct: Ecol\lacZ expression driven from an Hsp70 minimal promoter and governed by tandemly arrayed, optimized UAS Scer\GAL4 binding sites.
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Brand and Perrimon, 1993, Muller, 1995)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference Scer\GAL4-targeted reporter; expression of Ecol\lacZ reflects action of enhancer/promoter elements driving GAL4 in the same cell. (Brand and Perrimon, 1993)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAS-lacZ.B} (Brand and Perrimon, 1993)
descendant(s)	P{UAS-lacZ.UW14} (Yoshihara and Ito, 2000) P{UAS-lacZ.UW23-1} (Yoshihara and Ito, 2000)
Comments

Stocks Listed in FlyBase ( 6 )
Bloomington	1777 w^; P{UAS-lacZ.B}Bg4-2-4b 1776 w¹¹¹⁸; P{UAS-lacZ.B}melt^Bg4-1-2 4409 y¹ w^; P{AyGAL4}25 P{UAS-lacZ.B}melt^Bg4-1-2/CyO, y⁺
Kyoto	106501 w^; P{UAS-lacZ.B}Bg4-2-4b 106500 w^; P{UAS-lacZ.B}melt^Bg4-1-2 107722 y¹ w^*; P{AyGAL4}25 P{UAS-lacZ.B}melt^Bg4-1-2/CyO, y⁺
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	5UAS-hsp70-lacZ (Brodu et al., 1999) cytoplasmic Β-galactosidase (Kazemi-Esfarjani and Benzer, 2002) G4/lacZ (Flores-Saaib et al., 2001) GAL4-mini-white (Muller, 1995) P[UAS-lacZ] (Park et al., 2004, Cadigan et al., 2002, Wing et al., 2001, Rousset et al., 2001, Wing et al., 1998, Fuse et al., 1994) P[UAS lacZ] (Boivin and Dura, 1998) P[w⁺;UAS.lacZ] (Takaesu et al., 2002) P[w⁺; UAS-lacZ] (Staehling-Hampton and Hoffmann, 1994) P[w^+mCGAL4-Hsp.PB] (Armstrong et al., 2002) P{UAS_G-lacZ} (An et al., 2000) P{UAS-lacZ.B} (Roman et al., 2001, FlyBase, 1996-, Vanderzwan-Butler et al., 2007) P{UAS-LacZ.B} (Ford et al., 2007) P{UAS-LacZ} (Liu et al., 2007) P{w⁺mC=UAS-lacZ.B} (Paladi and Tepass, 2004) P{w^+mC=UAS-lacZ.B} (Velichkova et al., 2010) P{w^+mC lacZ^UAS.cBa=UAS-lacZ.B} (FlyBase, 1996-) P{w^+mCUAS::lac-Z} (Miller et al., 2001) P^GALBS-lacZ (Bejarano et al., 2005) P^UAS-lacZ (Chang et al., 2008) UAS:lacZ (Rincon-Limas et al., 1999) UAS/lacZ (Frasch, 1995) UAS.lacZ (Marquez et al., 2001) UAS_G-lacZ (Yang et al., 2000, Kramer et al., 1995, Armstrong et al., 1998) UAS-clacZ (Tokusumi et al., 2007) UAS-lacZ (Tsai et al., 2004, Monastirioti, 2003, Johnson et al., 2003, Wang et al., 2003, Gendre et al., 2004, Suzanne et al., 2003, Robertson et al., 2003, Savarit and Ferveur, 2002, Englund et al., 2002, Nakagoshi et al., 2002, Iwaki and Lengyel, 2002, Heimbeck et al., 2001, Klapper et al., 2002, Renaud and Simpson, 2001, Sepp et al., 2001, Klapper et al., 2001, Stortkuhl and Kettler, 2001, Dubois et al., 2001, Venkatesh et al., 2001, Yoshihara and Ito, 2000, Sandrelli et al., 2001, Furriols and Bray, 2000, Kolonin and Finley, 2000, Zhao et al., 2000, Klapper, 2000, Deuring et al., 2000, Kaneko and Hall, 2000, Park et al., 2000, Cooper et al., 2000, Feany and Bender, 2000, Balakireva et al., 2000, Chang et al., 2000, McBride et al., 1999, Han et al., 2000, Chao and Nagoshi, 1999, Renn et al., 1999, Namba and Minden, 1999, Sanson et al., 1999, Heimbeck et al., 1999, Kopp et al., 1999, de Celis et al., 1999, Jennings et al., 1999, Lee and Luo, 1999, Varadarajan and VijayRaghavan, 1999, Moreau-Fauvarque et al., 1998, Rorth, 1998, Akiyama-Oda et al., 1998, Allen et al., 1998, Brand and Perrimon, 1993, Baylies et al., 1995, Michelson, 1994, Lei et al., 2003, Kennerdell and Carthew, 2000, Apitz et al., 2005, Kim et al., 2006, Nguyen et al., 2007, Pereanu et al., 2007, Guan et al., 2007, Chan et al., 2007, Duan et al., 2007, Wolfstetter et al., 2009, Poirier et al., 2008, Wang and Huang, 2009, Barrett et al., 2008, Paladi and Tepass, 2004, Tokusumi et al., 2007, Marinho et al., 2011, Sellami et al., 2010, Otsuna and Ito, 2006, Jacobsson et al., 2009, Yang et al., 2011, Deng et al., 2010, Mershin et al., 2004, Wang et al., 2011) UAS-LacZ (Manoli and Baker, 2004, Sung and Robinow, 2000, Torroja et al., 1999, Gorfinkiel et al., 1999, Lahr et al., 2012, de Haro et al., 2006) UASlacZ (Holz et al., 2003, Fang et al., 2006, Benna et al., 2010) uaslacZ (Featherstone et al., 2000) UAS-lacz (Rhiner et al., 2010) UASlacz (Rhiner et al., 2010) UAS-lacZ 4.2.1 (Scholz et al., 2000) UAS-lacZ.B (Apitz et al., 2004, Kazemi-Esfarjani and Benzer, 2002, Jean et al., 2012) UAS-lacz(cytoplasmic) (Hu and Castelli-Gair, 1999) UAS-lacZ^1-71 (Harden et al., 2002) UAS-β-gal (Kidd and Lieber, 2002)
Secondary FlyBase IDs

References ( 233 )

Generate a list of	All references relating to this transgenic transposon ( 233 )

List References by type	Research paper ( 223 ) Review ( 3 ) Erratum ( 1 ) Personal communication to FlyBase ( 5 ) Abstract ( 1 )
Recent research papers ( 5 )
	Jean et al., 2012, Mol. Biol. Cell 23(14): 2723--2740 Sbf/MTMR13 coordinates PI(3)P and Rab21 regulation in endocytic control of cellular remodeling. [FBrf0218866] Lahr et al., 2012, J. Neurosci. 32(20): 6819--6829 Genetic analysis of ecdysis behavior in Drosophila reveals partially overlapping functions of two unrelated neuropeptides. [FBrf0218345] Marinho et al., 2011, Development 138(2): 349--357 The Drosophila Nol12 homologue viriato is a dMyc target that regulates nucleolar architecture and is required for dMyc-stimulated cell growth. [FBrf0212565] Wang et al., 2011, J. Neurosci. 31(8): 2868--2877 Protein misfolding and oxidative stress promote glial-mediated neurodegeneration in an alexander disease model. [FBrf0213281] Yang et al., 2011, Neuron 72(2): 285--299 Nak regulates localization of clathrin sites in higher-order dendrites to promote local dendrite growth. [FBrf0216477] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...