Recombinant construct P{UAS-Tgfβ-60A.S2}

General Information
Symbol	P{UAS-Tgfβ-60A.S2}	FlyBase ID	FBtp0000368
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Staehling-Hampton et al., 1994)
Uses	CV term Qualifiers & info Reference binary system (targeted) gbb (Staehling-Hampton et al., 1994) Tgfbeta-60A (Staehling-Hampton et al., 1994) characterization Tgfbeta-60A (Staehling-Hampton et al., 1994)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Staehling-Hampton et al., 1994) chimeric gene (Staehling-Hampton et al., 1994) selectable marker (Staehling-Hampton et al., 1994)
Component Alleles
Allele	gbb^Scer\UAS.cSb
Reference(s)	(Staehling-Hampton et al., 1994, Wharton, 2003.10.8, Miguel-Aliaga et al., 2004, Allan et al., 2003)
Molecular data	Construct: gbb EcoRI fragment cDNA (FBrf0055818), without an SV40 poly(A) signal, is under the transcriptional control of Scer\GAL4 UAS binding sites. (Staehling-Hampton et al., 1994)
Phenotypic class	lethal \| pupal stage, with Scer\GAL4^how-24B (Staehling-Hampton et al., 1994) visible, with Scer\GAL4^dpp.blk1 (Staehling-Hampton et al., 1994)
Phenotype manifest in	wing, with Scer\GAL4^dpp.blk1 (Staehling-Hampton et al., 1994)
Other information

Allele	w^+mC
Reference(s)	(Staehling-Hampton et al., 1994)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAS-Tgfβ-60A.S2} (Staehling-Hampton et al., 1994)
descendant(s)
Comments
	report comment: SV40 termination signals have been removed from the Tgfβ-60A.S^UAS.cSa allele in pP{UAS-Tgfβ-60A.S1}. Observed to produce stronger phenotypes. (Staehling-Hampton et al., 1994)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-Tgfβ-60A.S2} (FlyBase, 1996-) P{w^+mC Tgfβ-60A^UAS.cSb=UAS-Tgfβ-60A.S2} (FlyBase, 1996-) UAS-gbb (Miguel-Aliaga et al., 2004)
Secondary FlyBase IDs

References ( 5 )
Research paper	Miguel-Aliaga et al., 2004, Development 131(23): 5837--5848 Independent roles of the dachshund and eyes absent genes in BMP signaling, axon pathfinding and neuronal specification. [FBrf0180178] Allan et al., 2003, Cell 113(1): 73--86 Specification of neuropeptide cell identity by the integration of retrograde BMP signaling and a combinatorial transcription factor code. [FBrf0158766] Khalsa et al., 1998, Development 125(14): 2723--2734 TGF-/BMP superfamily members, gbb-60A and dpp, cooperate to provide pattern information and establish cell identity in the Drosophila wing. [FBrf0103295] Staehling-Hampton et al., 1994, Cell Growth Diffn 5(6): 585--593 Specificity of bone morphogenetic protein-related factors: Cell fate and gene expression changes in Drosophila embryos induced by decapentaplegic but not 60A. [FBrf0074522]
Personal communication to FlyBase	Wharton, 2003.10.8, UAS-gbb lines. UAS-gbb lines. [FBrf0173062]