FlyBase Recombinant Construct Report: P{UAS-cRaf1.gof}

General Information

Symbol

P{UAS-cRaf1.gof}

FlyBase ID

FBtp0000374

Feature type

transgenic_transposable_element

Component Allele(s)

Hsap\RAF1^gof.UAS

Size

Expression Data

Associated insertion(s)

2 available

Molecular map

Description and Uses

Location-dependent role

Description

CV term

Qualifiers and info

Reference

transposon

P-element

(Brand, 1997.6.30, Brand and Perrimon, 1994)

Construct components

Product class / Tool use(s)

Marker(s)

w^+mC

Component allele Hsap\RAF1^gof.UAS

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Description

Gain of function form of Hsap\RAF1 coding sequence is expressed under the influence of UASt regulatory sequences.

(Brand and Perrimon, 1994)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Features

CV term

Qualifiers and info

Reference

binary

component

(Brand, 1997.6.30)

binary

component

(Brand and Perrimon, 1994)

chimeric

gene

(Brand, 1997.6.30)

chimeric

gene

(Brand and Perrimon, 1994)

modified

gene

(Brand, 1997.6.30)

modified

gene

(Brand and Perrimon, 1994)

selectable

marker

(Brand, 1997.6.30, Brand and Perrimon, 1994)

Component Alleles

Allele

Hsap\RAF1^gof.UAS

Reference(s)

(Yu et al., 2015, Markstein et al., 2014, Roti et al., 2013, Yu et al., 2013, Sinenko and Mathey-Prevot, 2004, Lee et al., 2003, Brand, 1997.6.30, Brand and Perrimon, 1994)

Molecular data

Gain of function form of Hsap\RAF1 coding sequence is expressed under the influence of UASt regulatory sequences.

(Brand and Perrimon, 1994)

Phenotype Data

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{UAS-cRaf1.gof}

(Brand and Perrimon, 1994)

descendant(s)

Comments

report comment: Human cRaf1, deletion aa 2-334 (constitutively active).

(Brand, 1997.6.30)

Stocks (2)

Bloomington

2074

P{UAS-cRaf1.gof}ra2, y¹ w^*

Kyoto

106654

P{UAS-cRaf1.gof}ra2, y¹ w^*

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

P{UAS-Hraf.gof}

(FlyBase, 1996-)

P{UAS-cRaf1.gof}

(FlyBase, 1996-)

RAF(gof)

(Roti et al., 2013)

UAS-Raf^gof

(Markstein et al., 2014)

UAS-cRaf1^gof

(Sinenko and Mathey-Prevot, 2004)

UAS-hRaf

(Lee et al., 2003)

UAS-hRaf^Act

(Yu et al., 2015)

UAS-human Raf^gofUAS-ΔcRaf1

(Brand and Perrimon, 1994)

p{UAS-hRaf^Act}

(Yu et al., 2015)

p{UAS-human Raf^Act}

(Yu et al., 2013)

Secondary FlyBase IDs

References (9)

Report Sections

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