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Recombinant construct P{GAL4-Hsp70.PB}

General Information
Symbol	P{GAL4-Hsp70.PB}	FlyBase ID	FBtp0000376
Feature type	transgenic_transposon	Created / Updated	1997-03-21/2001-12-17
Size		Expression data	GAL4 reporter/driver
Location-dependent role		Associated insertions	12 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Brand et al., 1994)
Uses	CV term Qualifiers & info Reference binary system (regulatory) Hsp70* (Brand et al., 1994)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end
Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Brand et al., 1994) chimeric gene (Brand et al., 1994) inducible promoter (Brand et al., 1994) general promoter (Brand et al., 1994) selectable marker (Brand et al., 1994) reporter gene (Brand et al., 1994)
Component Alleles
Scer\GAL4hs.PB
Reference(s)	(Lung et al., 2002, Nagaraj and Banerjee, 2007, Fei et al., 2002, Banerjee et al., 2004, Liu et al., 2007, Liu and Lehmann, 2006, Langevin et al., 2005, Hwang et al., 2002, Chern and Choi, 2002, Huang et al., 2002, Cha et al., 2005, Tettweiler et al., 2005, Gottar et al., 2002, Chanut et al., 2002, Chen et al., 2002, Song and Xie, 2003, Cox et al., 2000, Rybak et al., 2002, Hedengren et al., 1999, Kanuka et al., 2005, Hoffman and Lis, 2000, Adam et al., 2003, Guha and Kornberg, 2005, Lai et al., 2000, Kenyon et al., 2005, Bittinger, 2004, Schober et al., 1999, Bessa and Casares, 2005, Tateno et al., 2000, Chen et al., 2002, Wan et al., 2000, Sang and Ready, 2002, Kanuka et al., 2005, Kleino et al., 2005, Juhasz et al., 2003, Miotto et al., 2006, Stocker et al., 2003, Saucedo et al., 2003, Calvi et al., 1998, Li et al., 2005, Quinn et al., 2001, Kramer et al., 2003, Chavous et al., 2001, Adachi-Yamada et al., 1999, Inoue et al., 2001, Dunne et al., 2002, Kozlova and Thummel, 2002, del Alamo et al., 2002, Couderc et al., 2002, Brown et al., 2003, Chang et al., 2001, Bergmann et al., 2002, Poux et al., 2002, Razzaq et al., 2001, Britton et al., 2002, Audibert et al., 2005, Bataille et al., 2005, Schulz et al., 2001, Lee et al., 2003, Matsuno et al., 2002, Tulin et al., 2006, Dai et al., 2003, Seong et al., 2002, Kondylis et al., 2001, Li and White, 2003, Ishikawa et al., 2005, Dostert et al., 2005, Szuplewski et al., 2003, Wolf et al., 2006, van de Bor et al., 2002, Colombani et al., 2005, Li et al., 1998, Ekengren et al., 2001, Baum and Perrimon, 2001, Kon, 2005, Liu et al., 2003, Harden et al., 2002, Mandal et al., 2007, Yamashita et al., 2007, Yamashita et al., 2007, Lebestky et al., 2003, Merianda et al., 2005, Senger et al., 2006, Park et al., 2004, Choi et al., 2004, Kim et al., 2006, Busygina et al., 2004, Jang et al., 2006, Fuss et al., 2006, Chihara et al., 2003, Seppo et al., 2003, Leulier et al., 2002, Solano et al., 2003, Zraly et al., 2003, Parker et al., 2002, Zheng et al., 2003, Cai et al., 2003, Satterfield et al., 2002, Labrador and Corces, 2003, French et al., 2003, Brand et al., 1993, Bardot et al., 2002, Enerly et al., 2002, Reichhart et al., 2002, Presente et al., 2002, Chan et al., 2002, Takehana et al., 2002, Song et al., 2002, Kuranaga et al., 2002, Mao et al., 2002, Jarman et al., 1993, Rodan et al., 2002, Savarit and Ferveur, 2002, Kiefer et al., 2002, Sen et al., 2004, Miura et al., 2002, Cheng and Shearn, 2004, Robinson et al., 2002, Duvic et al., 2002, Wang et al., 2004, Gu et al., 1995, Taylor et al., 1995, Ogden et al., 2003, Tsai et al., 2004, Armstrong et al., 2002, Acharya et al., 2004, Griffitts et al., 2003, Mason et al., 2002, Bai and Montell, 2002, Yu et al., 2004, Dejardin and Cavalli, 2004, Igaki et al., 2002, Karagiosis and Ready, 2004, Frankfort and Mardon, 2004, Enerly et al., 2003, Song et al., 2004, Kawase et al., 2004, Narbonne et al., 2004, Ikeya et al., 2002, Fuss et al., 2004, Bajpai et al., 2004, Brand et al., 1994, Martin and Baehrecke, 2004, Yamamoto and Nakano, 1999, Broughton et al., 2004, Wesley and Saez, 2000, Chen et al., 2004, LeBlanc et al., 1999, Nishimura et al., 2004, Chang et al., 2003, Gobert et al., 2003, Wasser and Chia, 2000, Serano and Rubin, 2003, DeSousa et al., 2003, Yang et al., 2000, Wittwer et al., 2001, Lee and Baehrecke, 2001, Brogiolo et al., 2001, Kumar and Moses, 2001, Tang et al., 2000, Kraus and Lis, 1994, Frolov et al., 2000, Roman et al., 2000, Noveen et al., 2000, Chang and Ready, 2000, Veraksa et al., 2000, Quinn et al., 2000, Uv et al., 2000, van de Bor et al., 2000, Jarman et al., 1994, Turnage et al., 2000, Ben-Arie et al., 2000, Decoville et al., 2001, Lefai et al., 2000, Ju et al., 2000, Barolo et al., 2000, Bloomington Drosophila Stock Center, 2000.3.11, Huang et al., 2000, Goulding et al., 2000, Steneberg et al., 1999, Cavalli, 1999, Rudolph et al., 1999, Dobens et al., 2000, Nie et al., 1999, Terhzaz et al., 1999, Lyko et al., 1999, Shi et al., 1999, Jarecki et al., 1999, Schaefer et al., 2000, Colussi et al., 2000, Toba et al., 1999, Savarit et al., 1999, Petersen et al., 1999, Hirose et al., 1999, Spencer et al., 1998, Brodu et al., 1999, zur Lage and Jarman, 1999, Kidd et al., 1998, Englund et al., 1999, Knoblich et al., 1999, Bhat et al., 1999, Heifetz et al., 2005, Harden et al., 1999, Kavaler et al., 1999, Macpherson et al., 2005, Huang et al., 1998, Ricos et al., 1999, Larkin et al., 1999, Li et al., 1999, Hayashi and Yamaguchi, 1999, Imam et al., 1999, Satoh et al., 2005, Li et al., 1997, Larsson and Rasmuson-Lestander, 1997, Ralston and Blair, 2005, Satoh et al., 1997, Szakmary et al., 2005, Hukriede et al., 1997, Kopp et al., 1997, Kopp and Duncan, 1997, Rosay et al., 1997, Riese et al., 1997, Zito et al., 1997, Song et al., 2000, Newfeld et al., 1997, Kolonin and Finley, 2000, Burgess et al., 1997, Wang et al., 2004, Vereshchagina et al., 2004, Porsch et al., 2005, Cordero et al., 2004, Tsai et al., 2004, Perazzona et al., 2004, Yao et al., 2006, Srinivasan and Atchison, 2004, Takehana et al., 2004, Adam and Montell, 2004, Brugger et al., 2004, Thomer et al., 2004, Conder et al., 2004, Woodcock and Hughes, 2004, Piccin et al., 2001, Lee et al., 2004, Simmonds et al., 2001, King et al., 2001, Chan et al., 2001, Bhat, 1998, Bacon et al., 1998, Axelrod et al., 1998, Gallet et al., 1998, van de Bor and Giangrande, 2001, Xie and Spradling, 1998, Cho et al., 2001, Khalsa et al., 1998, Roote and Zusman, 1996, Rousset et al., 2001, Lee et al., 1996, Winter et al., 2001, Betz et al., 2001, Tomoyasu et al., 1998, Mozer, 2001, Dobens et al., 2001, Jarman and Ahmed, 1998, Murphy and Montell, 1996, Frolov et al., 2001, Zaffran et al., 1998, Vidal et al., 2001, Forbes et al., 1996, Nakagoshi et al., 1998, Broughton et al., 1996, Ferveur and Savarit, 2000, Bokor and DiNardo, 1996, Brown and Freeman, 2003, Tata and Hartley, 1995, Hediger et al., 2004, McCall and Bender, 1996, Chen and McKearin, 2003, Zink and Paro, 1995, Fietz et al., 1995, Wines et al., 1996, Kozlova and Thummel, 2003, Harrison et al., 1995, Boettner et al., 2003, Hou et al., 1997, Kockel et al., 1997, Fleming et al., 1997, Kunert et al., 2003, Pai et al., 1997, Rose et al., 1997, Lee and Montell, 1997, Cavalli and Paro, 1998, Ferveur et al., 1997, Eresh et al., 1997, Roignant et al., 2003, Lai, 2003, Larkin et al., 1996, Riese et al., 1997, Martin-Blanco et al., 1998, Shen and Mardon, 1997, Kane et al., 1997, Zecca et al., 1996, Brunner et al., 1997, Freeman, 1996, Dokucu et al., 1996, Torok et al., 1997, Chien et al., 1996, Calleja et al., 1996, Howes et al., 1998, Sutherland et al., 1996, Davis et al., 1998, Yang et al., 2003, Seroude et al., 2002, Shin, 2005, Katsuyama et al., 2005, Park et al., 2006, Lu et al., 2006, Walker et al., 2006, Vrailas et al., 2006, Zelhof et al., 2006, Petruk et al., 2006, Kocks et al., 2005, Sanchez-Elsner et al., 2006, Kim et al., 2006, Cha et al., 2003, Jauch et al., 2006, Laviolette et al., 2005, Park et al., 2006, Kent et al., 2006, Senoo-Matsuda et al., 2005, Wesley and Mok, 2003, Tenney et al., 2006, Bainton et al., 2005, Takaesu et al., 2006, Papaconstantinou et al., 2005, Gottar et al., 2006, Matova and Anderson, 2006, Brennan et al., 2007, Serpe and O'Connor, 2006, Capelson and Corces, 2005, Das et al., 2006, Meyer and Aberle, 2006, Song et al., 2007, Chung et al., 2007, Sugiyama et al., 2007, Sano et al., 2005, Mueller et al., 2007)
Molecular data	GAL4 driven by an Hsp70 promoter. (Jarman et al., 1993) Expression of GAL4 driven by an heat inducible promoter. (Brand et al., 1993) Complete Scer\GAL4 coding sequence expressed from an Hsp70 promoter. (Brand et al., 1994) Construct: Scer\GAL4 is expressed from an Hsp70 promoter. (Forbes et al., 1996) A heat shock promoter drives Scer\GAL4 expression. (Torok et al., 1997) Scer\GAL4 is expressed under the control of a heat shock promoter. (Brunner et al., 1997, Rose et al., 1997)
Phenotypic class	viable, with Acp32CDScer\UAS.cLa (Lung et al., 2002) feeding behavior defective | larval stage, with sNPFScer\UAS.cLa (Lee et al., 2004) visible, with ato::scb.ato.Scer\UAS (Chien et al., 1996) lethal, with RhoLV20.Scer\UAS (Murphy and Montell, 1996) visible, with atoScer\UAS.cJa (Huang et al., 2000) long lived, with POSHGS2207 (Seong et al., 2002) viable, with Rac1N17.Scer\UAS (Murphy and Montell, 1996) lethal, with DIP1b.Scer\UAS (DeSousa et al., 2003) lethal, with DrefCR1.Scer\UAS (Hirose et al., 1999) melanotic 'tumor', with DrefCR1.Scer\UAS (Hirose et al., 1999) lethal, with DIP1c.Scer\UAS (DeSousa et al., 2003) visible, with atoScer\UAS.cCa (Chien et al., 1996) long lived, with klgGS0009 (Seong et al., 2002) viable, with Acp95EFScer\UAS.cLa (Lung et al., 2002) long lived, with CG31122GS0031 (Seong et al., 2002) lethal | larval stage | conditional - heat sensitive, with Csp2.Scer\UAS (Nie et al., 1999) visible, with ato::scHLH.sc.Scer\UAS (Chien et al., 1996) body color defective, with ydsRNA.Scer\UAS (Piccin et al., 2001) viable, with Cdc42N17.Scer\UAS (Murphy and Montell, 1996) visible, with B52Scer\UAS.cKa (Shi et al., 1999) visible | female, with Mdoa\dsxM1.Scer\UAS (Hediger et al., 2004) lethal, with SerBd.Scer\UAS.T:Hsap\MYC (Hukriede et al., 1997) visible, with ato::scbHLH.sc.Scer\UAS (Chien et al., 1996) long lived, with Hsp26GS3042 (Seong et al., 2002) visible, with amosScer\UAS.cGa (Goulding et al., 2000, Lai, 2003) visible | conditional - heat sensitive, with amosScer\UAS.cGa (Chanut et al., 2002) cell death increase, with Eip93FScer\UAS.cLa (Lee and Baehrecke, 2001) lethal, with Eip93FScer\UAS.cLa (Lee and Baehrecke, 2001) neurophysiology defective | conditional - heat sensitive, with eagΔ932.Scer\UAS (Broughton et al., 2004) hyperactive | conditional - heat sensitive, with eagΔ932.Scer\UAS (Broughton et al., 2004) viable, with DgkεScer\UAS.cFa (Frolov et al., 2001) fertile, with DgkεScer\UAS.cFa (Frolov et al., 2001) viable, with su(s)Scer\UAS.cTa (Turnage et al., 2000) cell death decrease | pupal stage, with Ncpro.C-A.Scer\UAS (Martin and Baehrecke, 2004) long lived, with GS2035GS2035 (Seong et al., 2002) long lived | male, with Hsp26Scer\UAS.T:Ivir\HA1 (Wang et al., 2004) chemical resistant | male, with Hsp26Scer\UAS.T:Ivir\HA1 (Wang et al., 2004) female fertile | reduced, with Hsp26Scer\UAS.T:Ivir\HA1 (Wang et al., 2004) viable, with Acp26AaScer\UAS.cPa (Lung et al., 2002) lethal | conditional - heat sensitive, with scScer\UAS.cCa (Huang et al., 2000) visible | conditional - heat sensitive, with scScer\UAS.cCa (Huang et al., 2000) visible, with scScer\UAS.cCa (Chien et al., 1996) lethal | embryonic stage, with SerScer\UAS.cGa (Fleming et al., 1997) lethal, with SerScer\UAS.cGa (Hukriede et al., 1997) semi-lethal, with Atx2Scer\UAS.cSa (Satterfield et al., 2002) lethal | larval stage, with spis.Scer\UAS (Freeman, 1996) long lived, with Df31GS2138 (Seong et al., 2002) cell death increase, with EgfrDN.Scer\UAS (Kurada and White, 1998) cell polarity defective, with EgfrDN.Scer\UAS (Brown and Freeman, 2003) cell polarity defective, with pon::rapsc-pon.Scer\UAS (Cai et al., 2003) lethal | partially, with fngScer\UAS.cKa (Fleming et al., 1997) visible, with amosScer\UAS.cHa (Huang et al., 2000) viable, with imdScer\UAS.cGa (Georgel et al., 2001) cell death increase, with imdScer\UAS.cGa (Georgel et al., 2001) radiation sensitive, with imdScer\UAS.cGa (Georgel et al., 2001) lethal, with Rac1V12.Scer\UAS (Murphy and Montell, 1996) feeding behavior defective | larval stage, with sNPFdsRNA.Scer\UAS (Lee et al., 2004) cell death increase, with debclScer\UAS.T:Ivir\HA1 (Colussi et al., 2000) lethal | embryonic stage | conditional - heat sensitive, with Csp1.Scer\UAS (Nie et al., 1999) viable, with Acp29ABScer\UAS.cLa (Lung et al., 2002) visible, with scbHLH.Scer\UAS (Chien et al., 1996) long lived, with Myo31DFGS2116 (Seong et al., 2002) lethal, with hopScer\UAS.cHa (Harrison et al., 1995) visible, with hopScer\UAS.cHa (Harrison et al., 1995) lethal, with Snr1cdel.3.Scer\UAS (Zraly et al., 2003) lethal, with NiPp1Scer\UAS.T:Avic\GFP-EGFP (Parker et al., 2002) long lived (Seong et al., 2002) long lived, with CG10157GS3028 (Seong et al., 2002) cell death increase, with aopACT.Scer\UAS (Kurada and White, 1998) short lived, with ThorY54A.M59A.Scer\UAS (Tettweiler et al., 2005) cell death decrease | conditional - heat sensitive, with Ras85DV12.Scer\UAS (Cordero et al., 2004) long lived, with NgpGS2207 (Seong et al., 2002) lethal | embryonic stage, with PatjScer\UAS.cBa (Bhat et al., 1999) visible, with LIMK1D522A.Scer\UAS (Chen et al., 2004) visible, with NECN.Scer\UAS (Barolo et al., 2000) melanotic 'tumor', with cadScer\UAS.cHa (Hwang et al., 2002) tissue polarity defective, with nkdScer\UAS.cZa (Rousset et al., 2001) lethal | embryonic stage | conditional - heat sensitive, with RpL14IR.dsRNA.Scer\UAS (Enerly et al., 2003) lethal | larval stage | conditional - heat sensitive, with RpL14IR.dsRNA.Scer\UAS (Enerly et al., 2003) lethal | conditional - heat sensitive, with RpL14IR.dsRNA.Scer\UAS (Enerly et al., 2003) Minute | conditional - heat sensitive, with RpL14IR.dsRNA.Scer\UAS (Enerly et al., 2003) cell polarity defective, with KrnScer\UAS.cUa (Brown and Freeman, 2003) cell death decrease | conditional - heat sensitive, with btl::EgfrScer\UAS.T:λ\cI-DD (Cordero et al., 2004) visible, with Mmus\Atoh1Scer\UAS.cBa (Ben-Arie et al., 2000) lethal, with mei-S332Scer\UAS.T:Avic\GFP-S65T (LeBlanc et al., 1999) viable, with Acp53EaScer\UAS.cLa (Lung et al., 2002) lethal | conditional - heat sensitive, with Acp62FScer\UAS.cLa (Lung et al., 2002) visible, with vri1.Scer\UAS (Szuplewski et al., 2003) viable, with Mmus\DnmtScer\UAS.cLa (Lyko et al., 1999) long lived, with CG8677GS2202 (Seong et al., 2002) lethal, with hopTum.Scer\UAS (Harrison et al., 1995) visible, with hopTum.Scer\UAS (Harrison et al., 1995) cell death increase, with NcScer\UAS.T:Avic\GFP-EGFP (Quinn et al., 2000) visible, with par-1Scer\UAS.cSa (Nishimura et al., 2004) neuroanatomy defective, with par-1Scer\UAS.cSa (Nishimura et al., 2004) long lived, with exuGS2115 (Seong et al., 2002) tissue polarity defective, with dshΔDEP+.Scer\UAS (Axelrod et al., 1998) lethal, with PsaScer\UAS.cSa (Schulz et al., 2001) tissue polarity defective, with dshΔbPDZ.Scer\UAS (Axelrod et al., 1998) viable | reduced, with Zzzz\aptamerB52.BBS.Scer\UAS (Shi et al., 1999) semi-lethal, with Zzzz\aptamerB52.BBS.Scer\UAS (Shi et al., 1999) lethal, with CrebB-17ACbz.Scer\UAS (Riese et al., 1997) lethal | embryonic stage | conditional - heat sensitive, with EcRB1-ΔC655.F645A.Scer\UAS (Kozlova and Thummel, 2003) long lived, with PcmtScer\UAS.cCa (Chavous et al., 2001) fertile, with PcmtScer\UAS.cCa (Chavous et al., 2001) locomotor behavior defective, with PcmtScer\UAS.cCa (Chavous et al., 2001) long lived, with sraGS3080 (Seong et al., 2002) viable, with Mmus\Dnmt3aScer\UAS.cLa (Lyko et al., 1999) semi-lethal, with bab24.Scer\UAS (Bardot et al., 2002) body color defective, with bab24.Scer\UAS (Couderc et al., 2002) long lived, with CG3308GS3107 (Seong et al., 2002) melanotic 'tumor' | conditional - heat sensitive, with PGRP-LEGS1068 (Takehana et al., 2002) lethal | conditional - heat sensitive, with PGRP-LEGS1068 (Takehana et al., 2002) long lived, with fs(2)ltoPP43GS2025 (Seong et al., 2002) visible | conditional - heat sensitive, with sogCR1-2.Scer\UAS (Yu et al., 2004) long lived, with Sema-5cGS3011 (Seong et al., 2002) visible, with HScer\UAS.cGa (Barolo et al., 2000) lethal, with PGRP-LEScer\UAS.T:Zzzz\FLAG (Takehana et al., 2004) visible, with putScer\UAS.cTa (Tomoyasu et al., 1998) immune response defective, with Tak1dsRNA.Scer\UAS (Leulier et al., 2002) long lived, with staiGS2209 (Seong et al., 2002) visible, with ato::scHLH.ato.Scer\UAS (Chien et al., 1996) locomotor behavior defective, with krzS047819, krzScer\UAS.cRa (Liu et al., 2007) neurophysiology defective | larval stage, with Pka-R1BGD.Scer\UAS (Davis et al., 1998) viable, with traScer\UAS.cFa (Savarit et al., 1999) courtship defective, with traScer\UAS.cFa (Savarit et al., 1999, Park et al., 2006) long lived, with CG6945GS3025 (Seong et al., 2002) lethal, with prodScer\UAS.cTa (Torok et al., 1997) semi-sterile, with prodScer\UAS.cTa (Torok et al., 1997) visible, with prodScer\UAS.cTa (Torok et al., 1997) lethal, with MYPT-75DHM.Scer\UAS (Vereshchagina et al., 2004) neurophysiology defective, with mthScer\UAS.cSa (Song et al., 2002) wild-type, with Hsap\KPNB1Scer\UAS.cKa (Kumar et al., 2001) semi-lethal, with bab1Scer\UAS.cGa (Bardot et al., 2002) lethal | conditional - heat sensitive, with CrebB-17Acor.Scer\UAS (Perazzona et al., 2004) memory defective, with CrebB-17Acor.Scer\UAS (Perazzona et al., 2004) visible, with Ilp2Scer\UAS.cBa (Brogiolo et al., 2001) large body, with Ilp2Scer\UAS.cBa (Brogiolo et al., 2001) increased cell number, with Ilp2Scer\UAS.cBa (Brogiolo et al., 2001)