Recombinant construct P{UAS-CD2}

General Information
Symbol	P{UAS-CD2}	FlyBase ID	FBtp0000378
Feature type	transgenic_transposon	Created / Updated	1997-03-21/2001-12-17

Size		Expression data
Location-dependent role		Associated insertions	2 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Dunin-Borkowski and Brown, 1995)
Uses	CV term Qualifiers & info Reference reporter construct Rnor\CD2 \| binary system (Dunin-Borkowski and Brown, 1995) binary system (targeted) Rnor\CD2 (Dunin-Borkowski and Brown, 1995) subcellular target cell surface (Dunin-Borkowski and Brown, 1995) characterization Scer\UAS (Dunin-Borkowski and Brown, 1995)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Dunin-Borkowski and Brown, 1995) chimeric gene (Dunin-Borkowski and Brown, 1995) selectable marker (Dunin-Borkowski and Brown, 1995) subcellularly targeted (Dunin-Borkowski and Brown, 1995)
Component Alleles
Rnor\CD2^{Scer\UAS.cDBa}
Reference(s)	(Grzeschik and Knust, 2005, Dunin-Borkowski and Brown, 1995, San Martin and Bate, 2001, Hummel et al., 2000, Martin-Bermudo et al., 1999, Sato et al., 1999, Galletta et al., 2004, Bachmann and Knust, 1998, Lecuit et al., 1996, Brand and Dormand, 1995, Scholz et al., 1997, Seugnet et al., 1997, Scholz et al., 1997, Huelsmann et al., 2006, Keene et al., 2006)
Molecular data	Construct: Expression of a ClaI XhoI fragment of Rnor\CD2 is influenced by KpnI CelII fragment carrying UAS Scer\GAL4 dependent promoter sequences and a SalI SpeI fragment containing the polyadenylation sequences of Hsp70. (Dunin-Borkowski and Brown, 1995)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{UAS-CD2} (Dunin-Borkowski and Brown, 1995)
descendant(s)
Comments
	construct_comment: Designed for use of the cell surface protein CD2 as a reporter gene; allows visualization of shape of expressing cell. (Dunin-Borkowski and Brown, 1995) construct_comment: Designed for use of the cell surface protein gene CD2 as a reporter gene; allows visualization of shape of expressing cell. (Dunin-Borkowski and Brown, 1995)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{@w^+m@ @CD2^UAS.cDBa@=@UAS-CD2@} (FlyBase, 1996-) P{UAS-CD2} (FlyBase, 1996-) P{w^+m CD2^UAS.cDBa=UAS-CD2} (FlyBase, 1996-) P{w^+m* CD2^UAS=UAS-CD2} (FlyBase, 1996-) UAS-CD2 (Grzeschik and Knust, 2005, Galletta et al., 2004, Hummel et al., 2000, Martin-Bermudo et al., 1999) UASCD2 (San Martin and Bate, 2001) UAS-cd2 (Huelsmann et al., 2006) uas-CD2 (Keene et al., 2006)
Secondary FlyBase IDs

References ( 16 )
Research paper	Huelsmann et al., 2006, Development 133(15): 2915--2924 The PDZ-GEF dizzy regulates cell shape of migrating macrophages via Rap1 and integrins in the Drosophila embryo. [FBrf0192108] Keene et al., 2006, Curr. Biol. 16(15): 1524--1530 Drosophila dorsal paired medial neurons provide a general mechanism for memory consolidation. [FBrf0193609] Grzeschik and Knust, 2005, Development 132(9): 2035--2045 IrreC/rst-mediated cell sorting during Drosophila pupal eye development depends on proper localisation of DE-cadherin. [FBrf0187462] Galletta et al., 2004, Mech. Dev. 121(12): 1455--1468 SNS: adhesive properties, localization requirements and ectodomain dependence in S2 cells and embryonic myoblasts. [FBrf0180484] San Martin and Bate, 2001, Development 128(2): 233--242 Hindgut visceral mesoderm requires an ectodermal template for normal development in Drosophila. [FBrf0132294] Hummel et al., 2000, Neuron 26(2): 357--370 Drosophila Futsch/22C10 is a MAP1B-like protein required for dendritic and axonal development. [FBrf0128497] Martin-Bermudo et al., 1999, Development 126(22): 5161--5169 Migration of the Drosophila primordial midgut cells requires coordination of diverse PS integrin functions. [FBrf0111966] Sato et al., 1999, Development 126(7): 1457--1466 Bar homeobox genes are latitudinal prepattern genes in the developing Drosophila notum whose expression is regulated by the concerted functions of decapentaplegic and wingless. [FBrf0108268] Bachmann and Knust, 1998, Mech. Dev. 76(1-2): 67--78 Positive and negative control of serrate expression during early development of the Drosophila wing. [FBrf0105192] Scholz et al., 1997, Mech. Dev. 62(1): 79--91 Control of midline glia development in the embryonic Drosophila CNS. [FBrf0093209] Seugnet et al., 1997, Development 124(10): 2015--2025 Transcriptional regulation of Notch and Delta: requirement for neuroblast segregation in Drosophila. [FBrf0093711] Lecuit et al., 1996, Nature 381(6581): 387--393 Two distinct mechanisms for long-range patterning by decapentaplegic in the Drosophila wing. [FBrf0087557] Dunin-Borkowski and Brown, 1995, Dev. Biol. 168(2): 689--693 Mammalian CD2 is an effective heterologous marker of the cell surface in Drosophila. [FBrf0080000]
Review	Brand and Dormand, 1995, Curr. Opin. Neurobiol. 5(5): 572--578 The GAL4 system as a tool for unravelling the mysteries of the Drosophila nervous system. [FBrf0084801]
Erratum	Scholz et al., 1997, Mech. Dev. 64(1-2): 137--151 Control of midline glia development in the embryonic Drosophila CNS. [FBrf0096245]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]