Recombinant construct P{per-lacZ.SG}

General Information
Symbol	P{per-lacZ.SG}	FlyBase ID	FBtp0000629
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Liu et al., 1988)
Uses	CV term Qualifiers & info Reference characterization per \| regulatory (Liu et al., 1988) reporter construct per (Liu et al., 1988)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric gene (Liu et al., 1988) molecular tag (Liu et al., 1988) reporter gene (Liu et al., 1988) selectable marker (Liu et al., 1988, Zwiebel et al., 1991)
Component Alleles
Allele	per^{SG.T:Ecol\lacZ}
Reference(s)	(Huang et al., 1993, Vosshall et al., 1994, Helfrich-Forster, 1995, Matsumoto et al., 1999, Liu et al., 1992, Zwiebel et al., 1991, Liu et al., 1991, Liu et al., 1988, Martinek et al., 2001, Price et al., 1998, Stanewsky et al., 1997, Dembinska et al., 1997, Cyran et al., 2005, Kaneko et al., 1997, Stanewsky et al., 2002)
Molecular data	Fusion between a fragment of per up to the SacI site within exon 5 and sequences encoding Ecol\lacZ. (Dembinska et al., 1997) A genomic per fragment containing 4.2kb 5' flanking sequences and part of the coding region (encoding the N-terminal 638 amino acids) is fused in frame to Ecol\lacZ. (Stanewsky et al., 1997) Fusion between a fragment of per and sequences encoding Ecol\lacZ. (Helfrich-Forster, 1995) Amino acids 1 to 636 and the 5' flanking sequences of per are fused upstream of the Ecol\lacZ coding region. (Vosshall et al., 1994) Construct: Fusion of per DNA including protein coding region up to within exon 5 (i.e. including PAS domain) to Ecol\lacZ coding region. (Huang et al., 1993) Fragment encoding the N-terminal 630 amino acids of per fused to Ecol\lacZ. (Liu et al., 1992) Contains the same extent of 5' flanking sequence and coding sequences as per^+t13.2 up to the SacI site in the 5th exon, fused to Ecol\lacZ. (Liu et al., 1991) 9kb fragment containing the 5' half of per transcribed DNA (encoding up to amino acid 607), and 4kb of per 5' upstream sequences, is fused to Ecol\lacZ. (Zwiebel et al., 1991) 4kb fragment of per 5' flanking sequences upstream of the transcription start site, the same extent as in per^+t13.2, and genomic sequences up to that encoding approximately the first 630 amino acids, within the fifth exon, are fused to Ecol\lacZ. (Liu et al., 1988)
Phenotypic class	locomotor rhythm defective (Stanewsky et al., 1997)
Phenotype manifest in
Other information

Allele	ry^+t7.2
Reference(s)	(Liu et al., 1988, Zwiebel et al., 1991, Liu et al., 1991)
Molecular data	Construct: 7.2kb HindIII fragment that contains ry⁺ function. (Rubin and Spradling, 1983)
Phenotypic class	wild-type (Rubin and Spradling, 1983)
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference larval stage larval DN1 neuron (Kaneko et al., 1997) larval DN2 neuron (Kaneko et al., 1997) larval DN3 neuron (Kaneko et al., 1997) larval LN period neuron (Kaneko et al., 1997) ring gland (Kaneko et al., 1997) mouth hooks (Kaneko et al., 1997) Comment:Expression is observed in tissue surrounding the mouth hook. ventral nerve cord \| subset (Kaneko et al., 1997) embryonic/larval brain \| ventral (Kaneko et al., 1997)
Additional Information
Statement Reference Expression was first seen in 12-14hr old first instar larvae. Expression was detected by X-gal histochemistry in DN2, but not DN1, in L2 larvae. Throughout larval development, expression is seen in cells which do not cycle per found in the ventral region of the brain. (Kaneko et al., 1997)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{per-lacZ.SG} (Liu et al., 1988)
descendant(s)
Comments
	report comment: In addition to 5\' regulatory sequences, the first 630 amino acids of per protein fused to lacZ coding region. (Liu et al., 1988)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{per-lacZ.630} (FlyBase, 1996-) P{per-lacZ.SG} (FlyBase, 1996-) per-lacZ (Matsumoto et al., 1999, Kaneko et al., 1997) SG (Kaneko et al., 1997) SG3 (Cyran et al., 2005) SG-lacZ (Stanewsky et al., 2002)
Secondary FlyBase IDs

References ( 15 )
Research paper	Cyran et al., 2005, J. Neurosci. 25(22): 5430--5437 The double-time protein kinase regulates the subcellular localization of the Drosophila clock protein period. [FBrf0187899] Stanewsky et al., 2002, J. Biol. Rhythms 17(4): 293--306 Mapping of elements involved in regulating normal temporal period and timeless RNA expression patterns in Drosophila melanogaster. [FBrf0151362] Martinek et al., 2001, Cell 105(6): 769--779 A role for the segment polarity gene shaggy/GSK-3 in the Drosophila circadian clock. [FBrf0136819] Matsumoto et al., 1999, Mol. Cell. Biol. 19(6): 4343--4354 tim^rit lengthens circadian period in a temperature-dependent manner through suppression of PERIOD protein cycling and nuclear localization. [FBrf0108911] Price et al., 1998, Cell 94(1): 83--95 double-time is a novel Drosophila clock gene that regulates PERIOD protein accumulation. [FBrf0103351] Dembinska et al., 1997, J. Biol. Rhythms 12(2): 157--172 Circadian cycling of a PERIOD-beta-galactosidase fusion protein in Drosophila: evidence for cyclical degradation. [FBrf0093087] Kaneko et al., 1997, J. Neurosci. 17(17): 6745--6760 Spatial and temporal expression of the period and timeless genes in the developing nervous system of Drosophila: Newly identified pacemaker candidates and novel features of clock gene product cycling. [FBrf0098279] Stanewsky et al., 1997, J. Neurosci. 17(2): 676--696 Temporal and spatial expression patterns of transgenes containing increasing amounts of the Drosophila clock gene period and a lacZ reporter: Mapping elements of the PER protein involved in circadian cycling. [FBrf0093737] Helfrich-Forster, 1995, Proc. Natl. Acad. Sci. U.S.A. 92(2): 612--616 The period clock gene is expressed in central nervous system neurons which also produce a neuropeptide that reveals the projections of circadian pacemaker cells within the brain of Drosophila melanogaster. [FBrf0078638] Vosshall et al., 1994, Science 263(5153): 1606--1609 Block in nuclear localization of period protein by a second clock mutation, timeless. [FBrf0068725] Liu et al., 1992, J. Neurosci. 12: 2735--2744 The period gene encodes a predominantly nuclear protein in adult Drosophila. [FBrf0056575] Liu et al., 1991, Neuron 6: 753--766 The strength and periodicity of Drosophila melanogaster circadian rhythms are differentially affected by alterations in period gene expression. [FBrf0054815] Zwiebel et al., 1991, Proc. Natl. Acad. Sci. U.S.A. 88: 3882--3886 A post-transcriptional mechanism contributes to circadian cycling of a per--galactosidase fusion protein. [FBrf0054972] Liu et al., 1988, Genes Dev. 2: 228--238 Spatial and temporal expression of the period gene in Drosophila melanogaster. [FBrf0048112]
Letter	Huang et al., 1993, Nature 364(6434): 259--262 PAS is a dimerization domain common to Drosophila period and several transcription factors. [FBrf0059155]