Recombinant construct P{Thb5-lacZ}

General Information
Symbol	P{Thb5-lacZ}	FlyBase ID	FBtp0000650
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Driever et al., 1989)
Uses	CV term Qualifiers & info Reference characterization bcd \| protein binding (Driever et al., 1989) reporter construct hb (Driever et al., 1989) synthetic DNA element bcd protein binding site(s) (Driever et al., 1989)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric gene (Driever et al., 1989) chimeric gene (Driever et al., 1989) reporter gene (Driever et al., 1989) selectable marker (Driever et al., 1989)
Component Alleles
Allele	Ecol\lacZ^hb.P5
Reference(s)	(Driever et al., 1989, Lopes et al., 2008, Holloway et al., 2011)
Molecular data	Construct: Ecol\lacZ gene expression is driven by a fragment of the hb upstream region, that carries all high and low affinity bcd binding sites and hb sequences upto position -1348, fused to Hsp70 promoter region. (Driever et al., 1989)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mW.hs
Reference(s)	(Driever et al., 1989)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage \| early organism \| 50-100% egg length (Driever et al., 1989) Comment:55-100% egg length
Additional Information
Statement Reference In early embryos, β-gal signal is strong. (Driever et al., 1989)

Marker for
Reflects expression of	hb (Driever et al., 1989)
Progenitors & Descendants
progenitor(s)	pP{Thb5-lacZ} (Driever et al., 1989)
descendant(s)
Comments
	construct_comment: One of a series of lacZ reporter constructs used to characterize hb 5\' regulatory sequences, including BCD binding sites. (Driever et al., 1989) construct_comment: One of a series of lacZ reporter constructs used to assay the efficacy ofthe bcd binding sites found within the 5\' regulatory region of hb@. (Driever et al., 1989)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	6B2H lacZ (Holloway et al., 2011) P{Thb5-lacZ} (FlyBase, 1996-) pThb5 (Driever et al., 1989, Lopes et al., 2008, Holloway et al., 2011)
Secondary FlyBase IDs

References ( 3 )
Research paper	Holloway et al., 2011, PLoS Comput. Biol. 7(2): e1001069 Gene Expression Noise in Spatial Patterning: hunchback Promoter Structure Affects Noise Amplitude and Distribution in Drosophila Segmentation. [FBrf0213002] Lopes et al., 2008, PLoS Comput. Biol. 4(9): e1000184 Spatial bistability generates hunchback expression sharpness in the Drosophila embryo. [FBrf0206086] Driever et al., 1989, Nature 340(6232): 363--367 Determination of spatial domains of zygotic gene expression in the Drosophila embryo by the affinity of binding sites for the bicoid morphogen. [FBrf0050639]